Coming of age: ten years of next-generation sequencing technologies

S Goodwin, JD McPherson, WR McCombie - Nature reviews genetics, 2016 - nature.com
Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …

Repetitive DNA and next-generation sequencing: computational challenges and solutions

TJ Treangen, SL Salzberg - Nature Reviews Genetics, 2012 - nature.com
Repetitive DNA sequences are abundant in a broad range of species, from bacteria to
mammals, and they cover nearly half of the human genome. Repeats have always …

Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic

M Vandenbogaert, A Kwasiborski, E Gonofio… - Scientific reports, 2022 - nature.com
Monkeypox is an emerging and neglected zoonotic disease whose number of reported
cases has been gradually increasing in Central Africa since 1980. This disease is caused by …

The present and future of de novo whole-genome assembly

J Sohn, JW Nam - Briefings in bioinformatics, 2018 - academic.oup.com
As the advent of next-generation sequencing (NGS) technology, various de novo assembly
algorithms based on the de Bruijn graph have been developed to construct chromosome …

Errors in long-read assemblies can critically affect protein prediction

M Watson, A Warr - Nature biotechnology, 2019 - nature.com
To the Editor—Long-read, single molecule sequencing technologies are now routinely used
to sequence and assemble the human genome1–3. These studies represent an important …

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett… - 2009 - academic.oup.com
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …

dDocent: a RADseq, variant-calling pipeline designed for population genomics of non-model organisms

JB Puritz, CM Hollenbeck, JR Gold - PeerJ, 2014 - peerj.com
Restriction-site associated DNA sequencing (RADseq) has become a powerful and useful
approach for population genomics. Currently, no software exists that utilizes both paired-end …

Towards achieving data security with the cloud computing adoption framework

V Chang, M Ramachandran - IEEE Transactions on services …, 2015 - ieeexplore.ieee.org
Offering real-time data security for petabytes of data is important for cloud computing. A
recent survey on cloud security states that the security of users' data has the highest priority …

Sequencing technologies—the next generation

ML Metzker - Nature reviews genetics, 2010 - nature.com
Demand has never been greater for revolutionary technologies that deliver fast, inexpensive
and accurate genome information. This challenge has catalysed the development of next …

[HTML][HTML] Assembly algorithms for next-generation sequencing data

JR Miller, S Koren, G Sutton - Genomics, 2010 - Elsevier
The emergence of next-generation sequencing platforms led to resurgence of research in
whole-genome shotgun assembly algorithms and software. DNA sequencing data from the …