The phasor plot: a universal circle to advance fluorescence lifetime analysis and interpretation

L Malacrida, S Ranjit, DM Jameson… - Annual Review of …, 2021 - annualreviews.org
The phasor approach to fluorescence lifetime imaging has become a common method to
analyze complicated fluorescence signals from biological samples. The appeal of the …

Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Biomolecular condensates modulate membrane lipid packing and hydration

A Mangiarotti, M Siri, NW Tam, Z Zhao… - Nature …, 2023 - nature.com
Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in
cell biology, playing an important role in a diverse range of processes across different …

Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane

M Amaro, F Reina, M Hof, C Eggeling… - Journal of physics D …, 2017 - iopscience.iop.org
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane
lipid packing can be achieved using polarity sensitive probes whose emission spectrum …

LAURDAN since Weber: the quest for visualizing membrane heterogeneity

G Gunther, L Malacrida, DM Jameson… - Accounts of chemical …, 2021 - ACS Publications
Conspectus Any chemist studying the interaction of molecules with lipid assemblies will
eventually be confronted by the topic of membrane bilayer heterogeneity and may ultimately …

The role of dielectrophoresis for cancer diagnosis and prognosis

GI Russo, N Musso, A Romano, G Caruso, S Petralia… - Cancers, 2021 - mdpi.com
Simple Summary Dielectrophoresis (DEP) is a label-free cell manipulation technique based
on electrical differences that can be applied to liquid biopsies in the context of cancer …

Pulmonary surfactant metabolism in the alveolar airspace: Biogenesis, extracellular conversions, recycling

B Olmeda, M Martínez-Calle, J Pérez-Gil - Annals of Anatomy …, 2017 - Elsevier
Pulmonary surfactant is a lipid–protein complex that lines and stabilizes the respiratory
interface in the alveoli, allowing for gas exchange during the breathing cycle. At the same …

Extracellular Vesicle Mobility in Collagen I Hydrogels Is Influenced by Matrix-Binding Integrins

NW Tam, A Becker, A Mangiarotti, A Cipitria… - ACS nano, 2024 - ACS Publications
Extracellular vesicles (EVs) are a diverse population of membrane structures produced and
released by cells into the extracellular space for the intercellular trafficking of cargo …

From mouse to human: Accessing the biochemistry of vision in vivo by two-photon excitation

G Palczewska, M Wojtkowski, K Palczewski - Progress in retinal and eye …, 2023 - Elsevier
The eye is an ideal organ for imaging by a multi-photon excitation approach, because ocular
tissues such as the sclera, cornea, lens and neurosensory retina, are highly transparent to …

Ratiometric fluorescence nanoscopy and lifetime imaging of novel Nile Red analogs for analysis of membrane packing in living cells

L Lauritsen, M Szomek, M Hornum, P Reinholdt… - Scientific Reports, 2024 - nature.com
Subcellular membranes have complex lipid and protein compositions, which give rise to
organelle-specific membrane packing, fluidity, and permeability. Due to its exquisite solvent …