Golden Standard: a complete standard, portable, and interoperative MoClo tool for model and non-model proteobacteria

B Blázquez, DS León, J Torres-Bacete… - Nucleic Acids …, 2023 - academic.oup.com
Modular cloning has become a benchmark technology in synthetic biology. However, a
notable disparity exists between its remarkable development and the need for …

[HTML][HTML] The Extraction, Biosynthesis, Health-Promoting and Therapeutic Properties of Natural Flavanone Eriodictyol

H Yin, Y Li, Y Feng, L Tian, Y Li - Nutrients, 2024 - mdpi.com
Eriodictyol is a flavanone compound commonly found in several edible plants. Ultrasound-
assisted extraction and high-performance liquid chromatography (HPLC) are commonly …

CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces

Y Lee, S Hwang, W Kim, JH Kim… - Journal of Industrial …, 2024 - academic.oup.com
The demand for discovering novel microbial secondary metabolites is growing to address
the limitations in bioactivities such as antibacterial, antifungal, anticancer, anthelmintic, and …

Metabolic engineering in Streptomyces albidoflavus for the biosynthesis of the methylated flavonoids sakuranetin, acacetin, and genkwanin

Á Pérez-Valero, S Ye, P Magadán-Corpas… - Microbial Cell …, 2023 - Springer
Flavonoids are important plant secondary metabolites showing antioxidant, antitumor, anti-
inflammatory, and antiviral activities, among others. Methylated flavonoids are particularly …

Metabolic engineering strategies for naringenin production enhancement in Streptomyces albidoflavus J1074

S Ye, P Magadán-Corpas, Á Pérez-Valero… - Microbial Cell …, 2023 - Springer
Background Naringenin is an industrially relevant compound due to its multiple
pharmaceutical properties as well as its central role in flavonoid biosynthesis. Results On …

Optimization of flavanonols heterologous biosynthesis in Streptomyces albidoflavus, and generation of auronols

P Magadán-Corpas, S Ye, A Braune, CJ Villar… - Frontiers in …, 2024 - frontiersin.org
Aromadendrin and taxifolin are two flavanonols (derived from the precursor naringenin)
displaying diverse beneficial properties for humans. The carbon skeleton of these flavonoids …

Modular Cloning Tools for Streptomyces spp. and Application to the De Novo Biosynthesis of Flavokermesic Acid

JM Massicard, D Noel, A Calderari… - ACS Synthetic …, 2024 - ACS Publications
The filamentous Streptomyces are among the most prolific producers of bioactive natural
products and are thus attractive chassis for the heterologous expression of native and …

Identification of a polyphenol O-methyltransferase with broad substrate flexibility in Streptomyces albidoflavus J1074

Á Pérez-Valero, P Magadán-Corpas, K Dulak… - Microbial Cell …, 2024 - Springer
Flavonoids are a large and important group of phytochemicals with a great variety of
bioactivities. The addition of methyl groups during biosynthesis of flavonoids and other …

Use of 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate Synthase (DAHP Synthase) to Enhance the Heterologous Biosynthesis of Diosmetin and Chrysoeriol in an …

Á Pérez-Valero, J Serna-Diestro, CJ Villar… - International Journal of …, 2024 - mdpi.com
Flavonoids are a large family of polyphenolic compounds with important agro-industrial,
nutraceutical, and pharmaceutical applications. Among the structural diversity found in the …

[HTML][HTML] Enhancing rufomycin production by CRISPR/Cas9-based genome editing and promoter engineering in Streptomyces sp. MJM3502

C Su, NQ Tuan, WH Li, JH Cheng, YY Jin… - Synthetic and Systems …, 2025 - Elsevier
Streptomyces sp. MJM3502 is a promising producer of rufomycins, which are a class of
potent anti-tuberculosis lead compounds. Although the structure, activity, and mechanism of …