Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …

The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current
sample preparation protocols, such as sample waste and lack of reproducibility. Here, we …

Air-water interface (AWI) interactions during cryo-electron microscopy (cryo-EM) sample
preparation cause significant sample loss, hindering structural biology research. Organisms …

Ice thickness is a critical parameter in single particle cryo-EM–too thin ice can break during
imaging or exclude the sample of interest, while ice that is too thick contributes to more …

Approximately two-thirds of the estimated one-billion metric tons of methane produced
annually by methanogens is derived from the cleavage of acetate. Acetate is broken down …

Single-particle cryoelectron microscopy (cryo-EM) continues to grow into a mainstream
structural biology technique. Recent developments in data collection strategies alongside …

Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures
of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; …

Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an
exceptionally well-suited technique for determining the structure of membrane proteins …

The specimen preparation process is a key determinant in the success of any cryo electron
microscopy (cryoEM) structural study and until recently had remained largely unchanged …