Structural variation, such as deletions, duplications, inversions and complex
rearrangements, can have profound effects on gene expression, genome stability …

Abstract Chimeric Antigen Receptor (CAR)-T-cell therapy has revolutionized cancer immune
therapy. However, challenges remain including increasing efficacy, reducing adverse events …

Prime editing (PE) enables precise and versatile genome editing without requiring double-
stranded DNA breaks. Here we describe the systematic optimization of PE systems to …

Abstract CRISPR–Cas9-mediated homology-directed repair (HDR) can introduce desired
mutations at targeted genomic sites, but achieving high efficiencies is a major hurdle in …

Prime editing installs precise edits into the genome with minimal unwanted byproducts, but
low and variable editing efficiencies have complicated application of the approach to high …

Mutational scanning connects genetic variants to phenotype, enabling the interrogation of
protein functions, interactions and variant pathogenicity. However, current methodologies …

The application of rapidly growing CRISPR toolboxes and methods has great potential to
transform biomedical research. Here, we provide a snapshot of up-to-date CRISPR …

We are often confronted with a simple question,“which gene editing technique is the best?”;
the simple answer is “there isn't one”. In 2021, a year after prime editing first made its mark …

Programmable gene integration technologies are an emerging modality with exciting
applications in both basic research and therapeutic development. Programmable addition …

Genetically encoded DNA recorders noninvasively convert transient biological events into
durable mutations in a cell's genome, allowing for the later reconstruction of cellular …