High-throughput and single-cell T cell receptor sequencing technologies
JA Pai, AT Satpathy - Nature methods, 2021 - nature.com
T cells express T cell receptors (TCRs) composed of somatically recombined TCRα and
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
TCRβ chains, which mediate recognition of major histocompatibility complex (MHC)–antigen …
Eleven grand challenges in single-cell data science
The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …
High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes
High-throughput single-cell RNA sequencing is a powerful technique but only generates
short reads from one end of a cDNA template, limiting the reconstruction of highly diverse …
short reads from one end of a cDNA template, limiting the reconstruction of highly diverse …
RNA interactions are essential for CTCF-mediated genome organization
R Saldaña-Meyer, J Rodriguez-Hernaez, T Escobar… - Molecular cell, 2019 - cell.com
The function of the CCCTC-binding factor (CTCF) in the organization of the genome has
become an important area of investigation, but the mechanisms by which CTCF dynamically …
become an important area of investigation, but the mechanisms by which CTCF dynamically …
Determining sequencing depth in a single-cell RNA-seq experiment
An underlying question for virtually all single-cell RNA sequencing experiments is how to
allocate the limited sequencing budget: deep sequencing of a few cells or shallow …
allocate the limited sequencing budget: deep sequencing of a few cells or shallow …
[HTML][HTML] Progress in multicellular human cardiac organoids for clinical applications
Advances in self-organizing cardiac organoids to recapitulate human cardiogenesis have
provided a powerful tool for unveiling human cardiac development, studying cardiovascular …
provided a powerful tool for unveiling human cardiac development, studying cardiovascular …
The scRNA-seq expression profiling of the receptor ACE2 and the cellular protease TMPRSS2 reveals human organs susceptible to SARS-CoV-2 infection
J Qi, Y Zhou, J Hua, L Zhang, J Bian, B Liu… - International Journal of …, 2021 - mdpi.com
COVID-19 patients always develop multiple organ dysfunction syndromes other than lungs,
suggesting the novel virus SARS-CoV-2 also invades other organs. Therefore, studying the …
suggesting the novel virus SARS-CoV-2 also invades other organs. Therefore, studying the …
AutoImpute: Autoencoder based imputation of single-cell RNA-seq data
The emergence of single-cell RNA sequencing (scRNA-seq) technologies has enabled us to
measure the expression levels of thousands of genes at single-cell resolution. However …
measure the expression levels of thousands of genes at single-cell resolution. However …
Comparing in vitro human liver models to in vivo human liver using RNA-Seq
R Gupta, Y Schrooders, D Hauser, M van Herwijnen… - Archives of …, 2021 - Springer
The liver plays an important role in xenobiotic metabolism and represents a primary target
for toxic substances. Many different in vitro cell models have been developed in the past …
for toxic substances. Many different in vitro cell models have been developed in the past …
An introduction to the analysis of single-cell RNA-sequencing data
The recent development of single-cell RNA sequencing has deepened our understanding of
the cell as a functional unit, providing new insights based on gene expression profiles of …
the cell as a functional unit, providing new insights based on gene expression profiles of …