Photocross-linking of nucleic acids to associated proteins

KM Meisenheimer, TH Koch - Critical reviews in biochemistry and …, 1997 - Taylor & Francis
Photocross-linking is a useful technique for the partial definition of the nucleic acid-protein
interface of nucleoprotein complexes. It can be accomplished by one or two photon …

Applications of aptamers in cancer cell biology

JA Phillips, D Lopez-Colon, Z Zhu, Y Xu, W Tan - Analytica chimica acta, 2008 - Elsevier
Identifying cells associated with specific disease states is critically important for the early
detection and diagnosis of cancer. To facilitate this task, molecular probes, which bind …

2′-Fluoropyrimidine RNA-based aptamers to the 165-amino acid form of vascular endothelial growth factor (VEGF165): Inhibition of receptor binding and VEGF …

J Ruckman, LS Green, J Beeson, S Waugh… - Journal of Biological …, 1998 - ASBMB
Vascular endothelial growth factor (VEGF) has been implicated in the pathological induction
of new blood vessel growth in a variety of proliferative disorders. Using the SELEX process …

Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers

MC Golden, BD Collins, MC Willis, TH Koch - Journal of biotechnology, 2000 - Elsevier
High sensitivity and specificity of two modified ssDNA aptamers capable of photocross-
linking recombinant human basic fibroblast growth factor (bFGF (155)) were demonstrated …

1, 3‐Diketone‐Modified Nucleotides and DNA for Cross‐Linking with Arginine‐Containing Peptides and Proteins

DL Leone, M Hubálek, R Pohl, V Sýkorová… - Angewandte …, 2021 - Wiley Online Library
Abstract Linear or branched 1, 3‐diketone‐linked thymidine 5′‐O‐mono‐and triphosphate
were synthesized through CuAAC click reaction of diketone‐alkynes with 5‐azidomethyl …

Squaramate‐modified nucleotides and DNA for specific cross‐linking with lysine‐containing peptides and proteins

I Ivancová, R Pohl, M Hubálek… - Angewandte Chemie …, 2019 - Wiley Online Library
Abstract Squaramate‐linked 2′‐deoxycytidine 5′‐O‐triphosphate was synthesized and
found to be good substrate for KOD XL DNA polymerase in primer extension or PCR …

Investigation of the Origin of the Sequence Selectivity for the 5-Halo-2 '-Deoxyuridine Sensitization of DNA to Damage by UV-Irradiation

T Chen, GP Cook, AT Koppisch… - Journal of the American …, 2000 - ACS Publications
A contrathermodynamic sequence selectivity (5 '-deoxyadenosine> 5 '-deoxyguanosine) for
UV-irradiation-induced strand damage in duplex DNA containing 5-bromo-2 '-deoxyuridine …

Oligonucleotide‐Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling

C Saintomé, O Monfret, G Doisneau… - …, 2024 - Wiley Online Library
A variety of proteins interact with DNA and RNA, including polymerases, histones,
ribosomes, transcription factors, and repair enzymes. However, the transient non‐covalent …

Analysis of protein–nucleic acid interactions by photochemical cross‐linking and mass spectrometry

H Steen, ON Jensen - Mass spectrometry reviews, 2002 - Wiley Online Library
Abstract I. Introduction 163 II. Photochemical Cross‐Linking 165 III. Structural Analysis of
Protein–Nucleic Acid Heteroconjugates 165 IV. Analytical Challenges 168 A. Ionization of …

Programming sp3 Quantum Defects along Carbon Nanotubes with Halogenated DNA

X Wu, M Kim, LJ Wang, AK Veetil… - Journal of the American …, 2024 - ACS Publications
Atomic defect color centers in solid-state systems hold immense potential to advance
various quantum technologies. However, the fabrication of high-quality, densely packed …