In vitro cell cultures are crucial research tools for modeling human development and
diseases. Although the conventional monolayer cell cultures have been widely used in the …

Pluripotent stem cells show a remarkable ability to self-organize and differentiate in vitro in
three-dimensional aggregates, known as organoids or organ spheroids, and to recapitulate …

The prognosis of patients with glioblastoma (GBM) remains dismal, with a median survival of
approximately 15 months. Current preclinical GBM models are limited by the lack of a" …

Three-dimensional (3D) culture systems have fueled hopes to bring about the next
generation of more physiologically relevant high-throughput screens (HTS). However …

Human brain development exhibits several unique aspects, such as increased complexity
and expansion of neuronal output, that have proven difficult to study in model organisms. As …

Microglia play critical roles in brain development, homeostasis, and disease. Microglia in
animal models cannot accurately model human microglia due to notable transcriptomic and …

Available methods for differentiating human embryonic stem cells (ESCs) and induced
pluripotent cells (iPSCs) into neurons are often cumbersome, slow, and variable …

Background Because of their differentiation potential, pluripotent stem cells can generate
virtually any cell type and, as such, can be used to model development and disease and …

Axonal regeneration and formation of tripartite (axo-glial) junctions at damaged sites is a
prerequisite for early repair of injured spinal cord. Transplantation of stem cells at such sites …

Recent breakthroughs in pluripotent stem cell technologies have enabled a new class of in
vitro systems for functional modeling of human brain development. These advances, in …