Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …

Conventional methods of DNA sequence insertion into plants, using Agrobacterium-
mediated transformation or microprojectile bombardment, result in the integration of the DNA …

The current technologies to place new DNA into specific locations in plant genomes are low
frequency and error-prone, and this inefficiency hampers genome-editing approaches to …

Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

Recently discovered bacteria and archaea adaptive immune system consisting of clustered
regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) …

The efficient repair of double-strand breaks (DSBs) in genomic DNA is important for the
survival of all organisms. In recent years, basic mechanisms of DSB repair in somatic plant …

Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

Targeted mutagenesis is an essential tool of reverse genetics that could be used
experimentally to investigate basic plant biology or modify crop plants for improvement of …

Site-directed mutagenesis in higher plants remains a significant technical challenge for
basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation …

Homologous recombination offers great promise for plant genome engineering. This
promise has not been realized, however, because when DNA enters plant cells homologous …