The construction of custom libraries is critical for rational protein engineering and directed
evolution. Array‐synthesized oligo pools of thousands of user‐defined sequences (up to∼ …

A general method to generate biosensors for user-defined molecules could provide
detection tools for a wide range of biological applications. Here, we describe an approach …

Highlights•The development of synthetic plant biosensors is progressing in model
plants.•More orthogonal protein scaffolds are needed to detect structurally diverse …

The potential emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
spike (S) escape mutants is a threat to the efficacy of existing vaccines and neutralizing …

Cancer cells have long been recognized to exhibit unique bioenergetic requirements. The
apoptolidin family of glycomacrolides are distinguished by their selective cytotoxicity towards …

The HIV-1 fusion peptide (FP) represents a promising vaccine target, but global FP
sequence diversity among circulating strains has limited anti-FP antibodies to~ 60 …

Antibodies are engineerable quantities in medicine. Learning antibody molecular
recognition would enable the in silico design of high affinity binders against nearly any …

Multiplexed assays of variant effects (MAVEs) have made possible the functional
assessment of all possible mutations to genes and regulatory sequences. A core pillar of the …

Protocols for the construction of large, deeply mutagenized protein encoding libraries via
Golden Gate assembly of synthetic DNA cassettes employ disparate, system‐specific …

High-throughput DNA fluorescence in situ hybridization (hiFISH) combines multicolor
combinatorial DNA FISH staining with automated image acquisition and analysis to visualize …