Highlights•Inducible gene regulation has been a critical tool in interrogating biological
systems.•Small-molecule induction enables regulation at the DNA, RNA, and protein …

Synthetic biologists have made great progress over the past decade in developing methods
for modular assembly of genetic sequences and in engineering biological systems with a …

While the installation of complex genetic circuits in microorganisms is relatively routine, the
synthetic biology toolbox is severely limited in plants. Of particular concern is the absence of …

Reliable, predictable engineering of cellular behavior is one of the key goals of synthetic
biology. As the field matures, biological engineers will become increasingly reliant on …

Abstract Motivation The LINCS L1000 project has collected gene expression profiles for
thousands of compounds across a wide array of concentrations, cell lines, and time points …

Antibiotics and their corresponding resistance genes act as a tool to control bacterial
survival. Antibiotic resistance is used to select for desired engineered cells, and study how …

Synthetic Notch receptor is flourishing as a powerful tool for the accurate control of cell
functions. It is a sensing receptor system developed based on the Notch signaling pathway …

Living cells perform sophisticated computations that guide them toward discrete states.
Synthetic genetic circuits are powerful tools for programing these computations, where …

Multicellular organisms perform sophisticated computations on a myriad of environmental
cues and produce many discrete cell states in response. These phenotypes are often …

Flow cytometry is a powerful quantitative assay supporting high-throughput collection of
single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with …