Macromolecular crowding is more than hard-core repulsions
Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
review the experimental evidence that crowding affects the equilibrium thermodynamics of …
Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications
J Sankaran, T Wohland - Communications Biology, 2023 - nature.com
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the
quantitative measurement of biomolecular dynamics and interactions. Improvements in …
quantitative measurement of biomolecular dynamics and interactions. Improvements in …
Influence of nonspecific interactions on protein associations: implications for biochemistry in vivo
The cellular interior is composed of a variety of microenvironments defined by distinct local
compositions and composition-dependent intermolecular interactions. We review the …
compositions and composition-dependent intermolecular interactions. We review the …
Toward an understanding of biochemical equilibria within living cells
Four types of environmental effects that can affect macromolecular reactions in a living cell
are defined: nonspecific intermolecular interactions, side reactions, partitioning between …
are defined: nonspecific intermolecular interactions, side reactions, partitioning between …
[HTML][HTML] Recent applications of fluorescence correlation spectroscopy in live systems
R Macháň, T Wohland - FEBS letters, 2014 - Elsevier
Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and
has helped address many questions in the life sciences. It provides important advantages …
has helped address many questions in the life sciences. It provides important advantages …
[HTML][HTML] Formation of protein complexes in crowded environments–from in vitro to in vivo
Y Phillip, G Schreiber - FEBS letters, 2013 - Elsevier
Traditionally, biochemical studies are performed in dilute homogenous solutions, which are
very different from the dense mixture of molecules found in cells. Thus, the physiological …
very different from the dense mixture of molecules found in cells. Thus, the physiological …
Protein-complex stability in cells and in vitro under crowded conditions
SS Stadmiller, GJ Pielak - Current Opinion in Structural Biology, 2021 - Elsevier
Highlights•Both steric repulsions and chemical interactions are important.•Systematic
experiments involving temperature, crowder molecular weight and concentration are …
experiments involving temperature, crowder molecular weight and concentration are …
Fluorescence correlation spectroscopy: an efficient tool for measuring size, size-distribution and polydispersity of microemulsion droplets in solution
Fluorescence correlation spectroscopy (FCS) is an ideal tool for measuring molecular
diffusion and size under extremely dilute conditions. However, the power of FCS has not …
diffusion and size under extremely dilute conditions. However, the power of FCS has not …
Computational Approaches to Predict Protein–Protein Interactions in Crowded Cellular Environments
Investigating protein–protein interactions is crucial for understanding cellular biological
processes because proteins often function within molecular complexes rather than in …
processes because proteins often function within molecular complexes rather than in …
Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy
YH Foo, N Naredi-Rainer, DC Lamb, S Ahmed… - Biophysical journal, 2012 - cell.com
Fluorescence cross-correlation spectroscopy (FCCS) is used to determine interactions and
dissociation constants (K ds) of biomolecules. The determination of a K d depends on the …
dissociation constants (K ds) of biomolecules. The determination of a K d depends on the …