Serine protease inhibitors in plants: nature's arsenal crafted for insect predators

F Jamal, PK Pandey, D Singh, MY Khan - Phytochemistry Reviews, 2013 - Springer
Plant serine protease inhibitors are defense proteins crafted by nature for inhibiting serine
proteases. Use of eco-friendly, sustainable and effective protein molecules which could halt …

Purification and characterization of a Bowman-Birk proteinase inhibitor from the seeds of black gram (Vigna mungo)

ER Prasad, A Dutta-Gupta, K Padmasree - Phytochemistry, 2010 - Elsevier
A proteinase inhibitor (BgPI) was purified from black gram, Vigna mungo (cv. TAU-1) seeds
by using ammonium sulfate fractionation, followed by ion-exchange, affinity and gel-filtration …

Development of a rapid process for purification of Bowman-Birk and Kunitz inhibitors from legume seeds, and evaluation of their biophysical, insecticidal, and …

M Gujjarlapudi, B Kotarya, SS Mohanraj… - International Journal of …, 2023 - Elsevier
Abstract Bowman-Birk inhibitor (BBI~ 10 kDa) and Kunitz inhibitor (KI~ 20 kDa) are serine
protease/proteinase inhibitor (s)[PI (s)] ubiquitously found in several Leguminous plant …

Bowman− Birk Proteinase inhibitor from Cajanus cajan seeds: purification, characterization, and insecticidal properties

ER Prasad, H Merzendorfer… - Journal of agricultural …, 2010 - ACS Publications
A red gram proteinase inhibitor (RgPI) was purified from red gram (Cajanus cajan) seeds by
using ammonium sulfate precipitation and ion-exchange, affinity, and gel filtration …

Purification and properties of a heat-stable enzyme of Pseudomonas fluorescens Rm12 from raw milk

Z Mu, M Du, Y Bai - European Food Research and Technology, 2009 - Springer
Pseudomonas fluorescens Rm12 is a kind of Psychrotrophic bacteria growing in cold raw
milk. It produced an extracellular heat resistant protease with an estimated molecular weight …

Stability of a black eyed pea trypsin chymotrypsin inhibitor (BTCI)

L da Silva, J SA Leite, C Bloch Jr… - Protein and Peptide …, 2001 - ingentaconnect.com
The stability of BTCI has been investigated as function of pH and temperature, following its
inhibitory activity against trypsin. The isolated inhibitor of 9,084 Da is stable over pH 3 to 10 …

Purification and primary structure determination of two Bowman–Birk type trypsin isoinhibitors from Cratylia mollis seeds

PMG Paiva, MLV Oliva, H Fritz, L Coelho… - Phytochemistry, 2006 - Elsevier
Two Bowman-Birk type trypsin inhibitors (CmTI1 and CmTI2) were purified from Cratylia
mollis seeds by acetone precipitation, ion exchange, gel filtration and reverse-phase …

Purification and characterization of a novel proteinase A inhibitor from Ganoderma lucidum by submerged fermentation

YP Tian, KC Zhang - Enzyme and Microbial Technology, 2005 - Elsevier
A novel proteinase A inhibitor was purified from Ganoderma lucidum. The purification was
carried out by ethanol precipitation (50–80%), ACA44 gel filtration and Source 30Q anion …

Mechanistic study on the anti‐proinflammatory activity of Kunitz type inhibitor from Caesalpinia decapetala seeds

SH Rajegowda, AH SnehaRani - Cell Biochemistry and …, 2023 - Wiley Online Library
The study reports the biochemical characterization and mechanism of action of a novel 19.6
kDa protease inhibitor (PIs) isolated from the seeds of Caesalpinia decapetala belonging to …

Trypsin Isoinhibitors with Antiproliferative Activity toward Leukemia Cells from Phaseolus vulgaris cv “White Cloud Bean”

J Sun, H Wang, TB Ng - BioMed Research International, 2010 - Wiley Online Library
A purification protocol that comprised ion exchange chromatography on DEAE‐cellulose,
affinity chromatography on Affi‐gel blue gel, ion exchange chromatography on SP …