Cryo-electron microscopy of vitrified specimens was just emerging as a practical method
when Richard Henderson proposed that we should teach an EMBO course on the new …

Radiation damage is the main problem which prevents the determination of the structure of a
single biological macromolecule at atomic resolution using any kind of microscopy. This is …

The aim of this book is to outline the physics of image formation, electron specimen
interactions and image interpretation in transmission electron mic roscopy. The book …

Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be
prepared for observation by cryo-electron microscopy in easily controlled conditions. The …

Accurate knowledge of defocus and tilt parameters is essential for the determination of three-
dimensional protein structures at high resolution using electron microscopy. We present two …

The first edition of this highly successful book appeared in 1975 and evolved from lecture
notes for classes in physical optics, diffraction physics and electron microscopy given to …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

Mature, infectious HIV‐1 particles contain a characteristic cone‐shaped core that encases
the viral RNA and replication proteins. The architectures of mature virions and isolated cores …

Three‐dimensional (3D) structural analysis is essential to understand the relationship
between the structure and function of an object. Many analytical techniques, such as X‐ray …

The projected structures of two unstained periodic biological specimens, the purple
membrane and catalase, have been determined by electron microscopy to resolutions of 7 Å …