The dynamic nature of the structure of monoclonal antibodies (mAbs) can be probed at a
resolution of 5-20 residues using hydrogen-deuterium exchange mass spectrometry (H/D …

Abstract i-Motifs (iMs) are non-canonical, four-stranded secondary structures formed by
stacking of hemi-protonated CH+· C base pairs in cytosine-rich DNA sequences …

Introduction: Forced degradation (FD) studies (stress testing) are an integral part of
pharmaceutical product development. Areas covered: The design and execution of these …

The nucleotide-free chaperonin GroEL is capable of capturing transient unfolded or partially
unfolded states that flicker in and out of existence due to large-scale protein dynamic …

Bacterial toxin or viral entry into the cell often requires cell surface binding and endocytosis.
The endosomal acidification induces a limited unfolding/refolding and membrane insertion …

An automated method using biotinylated GroEL-streptavidin biosensors with biolayer
interferometry (GroEL-BLI) was evaluated to detect the formation of transiently formed …

In vivo, proteins are often part of large macromolecular complexes where binding specificity
and dynamics ultimately dictate functional outputs. In this work, the pre-endosomal anthrax …

Currently, anticoagulants would be used to prevent thrombosis. Thrombin is an effector
enzyme for haemostasis and thrombosis. We designed a direct thrombin inhibitor peptide …

Horseradish peroxidase (HRP) isoenzyme C1a is one of the most widely used enzymes for
various analytical methods in bioscience research and medical fields. In these fields, real …

Stabilizing the folded state of metastable and/or aggregation-prone proteins through
exogenous ligand binding is an appealing strategy for decreasing disease pathologies …