Electroporation designates the use of short high‐voltage pulses to overcome the barrier of
the cell membrane. By applying an external electric field, which just surpasses the …

The use of electric pulses to transfect all types of cells is well known and regularly used in
vitro for bacteria and eukaryotic cells transformation. Electric pulses can also be delivered in …

Abstract Interleukin-12 (IL-12) has been used in numerous immunotherapy protocols against
melanoma. However, delivery of IL-12 in the form of recombinant protein can result in severe …

It is generally recognized that DNA vaccines are often less effective in large animals than in
mice. One possible reason for this reduced effectiveness may be transfection efficiency and …

In vivo electroporation (EP) is a versatile delivery method for gene transfer which can be
applied to any accessible tissue. Delivery of plasmid DNA encoding therapeutic genes or …

Electroporation designates the use of electric pulses to transiently permeabilize the cell
membrane. It has been shown that DNA can be transferred to cells through a combined …

Interest in drug delivery systems is growing as the delivery of many drugs continues to be a
problem due to safety, stability, and compliance issues. This is especially true for the …

Electrochemotherapy combined with peritumoral interleukin‐12 (IL‐12) gene electrotransfer
was used for treatment of mast cell tumours in 18 client‐owned dogs. Local tumour control …

Electrogene therapy combines administration of plasmid DNA into tissue followed by local
application of electric pulses. In electrogene therapy with interleukin-12 (IL-12), different …

In recent years, synthetic mRNA-based applications to produce desired exogenous proteins
in cells have been gaining importance. However, systemic delivery of synthetic mRNA can …