Biomolecular assembly is a key driving force in nearly all life processes, providing structure,
information storage, and communication within cells and at the whole organism level. These …

Highlights•Transcription Factors represent a starting material of choice to build new
biosensors.•Evolution and rational design are making advances toward tailor-made …

Biological nitrogen fixation plays a crucial role in the nitrogen cycle. An ability to fix
atmospheric nitrogen, reducing it to ammonium, was described for multiple species of …

Amino acid substitutions at nonconserved protein positions can have noncanonical and
“long-distance” outcomes on protein function. Such outcomes might arise from changes in …

Advances in personalized medicine and protein engineering require accurately predicting
outcomes of amino acid substitutions. Many algorithms correctly predict that evolutionarily …

Interpreting changes in patient genomes, understanding how viruses evolve and
engineering novel protein function all depend on accurately predicting the functional …

Recent progress in DNA synthesis and sequencing technology has enabled systematic
studies of protein function at a massive scale. We explore a deep mutational scanning study …

When amino acids vary during evolution, the outcome can be functionally neutral or
biologically‐important. We previously found that substituting a subset of nonconserved …

In vitro experimental evolution has taught us many lessons on the molecular bases of
adaptation. To move towards more natural settings, evolution in the mice gut has been …

Rheostat positions, which can be substituted with various amino acids to tune protein
function across a range of outcomes, are a developing area for advancing personalized …