The in vitro production of porcine embryos has presented numerous challenges to
researchers over the past four decades. Some of the problems encountered were specific to …

Initial technologies for creating transgenic swine only permitted random integration of the
construct. However, by combining the technology for homologous recombination in fetal …

Developing sustainable food systems is essential, especially for emerging economies,
where food systems are changing rapidly and affect the environment and natural resources …

It is now over a decade since the birth, in 1996, of Dolly the first animal to be produced by
nuclear transfer using an adult derived somatic cell as nuclear donor. Since this time similar …

Porcine embryonic fibroblasts (PEF) are important as donor cells for nuclear transfer for
generation of genetically modified pigs. In this study, we determined an optimal protocol for …

Contents Recent advances in new technologies to produce cloned and genetically modified
pigs involve manipulating oocytes and/or embryos in vitro. Although a great deal of progress …

The genetics of the pig: Pigs as a model for biomedical sciences. Page 1 ©CAB International
2011. The Genetics of the Pig, 426 2nd Edn (eds MF Rothschild and A. Ruvinsky) Kristy N …

The objective of the present work was to investigate and clarify the factors affecting the
efficiency of somatic cell nuclear transfer (NT) in the horse, including embryo reconstruction …

Background Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells
is currently the most widely used strategy to generate tailored pig models for biomedical …

In mice and other mammals, spermatogenesis is maintained by spermatogonial stem cells
(SSCs), a cell population belonging to undifferentiated type A spermatogonia. In the …