Hypervirulent R20291 Clostridioides difficile spores show disinfection resilience to sodium hypochlorite despite structural changes

D Malyshev, IA Jones, M McKracken, R Öberg… - BMC microbiology, 2023 - Springer
Background Clostridioides difficile is a spore forming bacterial species and the major
causative agent of nosocomial gastrointestinal infections. C. difficile spores are highly …

A lab-on-a-chip utilizing microwaves for bacterial spore disruption and detection

S Valijam, DPG Nilsson, R Öberg… - Biosensors and …, 2023 - Elsevier
Bacterial spores are problematic in agriculture, the food industry, and healthcare, with the
fallout costs from spore-related contamination being very high. Spores are difficult to detect …

The role of endospore appendages in spore–spore interactions in the pathogenic Bacillus cereus group

UL Jonsmoen, D Malyshev, M Sleutel… - Environmental …, 2024 - Wiley Online Library
Species within the Bacillus cereus sensu lato group, known for their spore‐forming ability,
are recognized for their significant role in food spoilage and food poisoning. The spores of B …

Monitoring bacterial spore metabolic activity using heavy water-induced Raman peak evolution

R Öberg, T Dahlberg, D Malyshev, M Andersson - Analyst, 2023 - pubs.rsc.org
Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection
in hospitals. Therefore, methods to monitor spore metabolic activity and verify sterilization …

Berberine-loaded nanoemulsions as a natural food preservative; the impact of femtosecond laser irradiation on the antibacterial activity

L Parseghian, N Kahrarian, AS Arabanian, ZM Alvand… - Heliyon, 2024 - cell.com
There is a growing concern among food safety regulators, the food industry, and consumers
about foodborne illnesses. To improve food safety and increase shelf life, it is necessary to …

Real-Time Monitoring of H2O2 Sterilization on Individual Bacillus atrophaeus Spores by Optical Sensing with Trapping Raman Spectroscopy

M Bertz, D Molinnus, MJ Schöning, T Homma - Chemosensors, 2023 - mdpi.com
Hydrogen peroxide (H2O2), a strong oxidizer, is a commonly used sterilization agent
employed during aseptic food processing and medical applications. To assess the …

Physico-chemical characterization of single bacteria and spores using optical tweezers

DPG Nilsson, UL Jonsmoen, D Malyshev… - Research in …, 2023 - Elsevier
Spore-forming pathogenic bacteria are adapted for adhering to surfaces, and their
endospores can tolerate strong chemicals making decontamination difficult. Understanding …

Assessing CaDPA levels, metabolic activity, and spore detection through deuterium labeling

R Öberg, TB Sil, A Ohlin, M Andersson, D Malyshev - Analyst, 2024 - pubs.rsc.org
Many strains among spore-forming bacteria species are associated with food spoilage,
foodborne disease, and hospital-acquired infections. Understanding the impact of …

Probing the Onset of the Bacterial Membrane Photodamage in Time using a Raman Optical Tweezer

AV Bhat, VR Soma, S Ananthamurthy - Vibrational Spectroscopy, 2023 - Elsevier
Laser-induced photo damage of the optically confined microorganism is known to affect the
bacterial membrane. We record the Raman spectra of a live, optically trapped Bacillus …

Evaluating Bacterial Spore Preparation Methods for Scanning Electron Microscopy

D Malyshev, CC Lee… - Microscopy and …, 2024 - academic.oup.com
Scanning electron microscopy (SEM) can reveal the ultrastructure of bacterial spores,
including morphology, surface features, texture, spore damage, germination, and …