SD Cole, AE Miklos, AC Chiao, ZZ Sun… - Synthetic and Systems …, 2020 - Elsevier
Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread …
Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal …
The de novo construction of a living organism is a compelling vision. Despite the astonishing technologies developed to modify living cells, building a functioning cell “from scratch” has …
MJ Hammerling, A Krüger, MC Jewett - Nucleic acids research, 2020 - academic.oup.com
Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights …
A Doerr, D Foschepoth, AC Forster, C Danelon - Scientific Reports, 2021 - nature.com
Abstract The Protein synthesis Using Recombinant Elements (PURE) system enables transcription and translation of a DNA template from purified components. Therefore, the …
A Doerr, E De Reus, P Van Nies… - Physical …, 2019 - iopscience.iop.org
DNA-guided cell-free protein synthesis using a minimal set of purified components has emerged as a versatile platform in constructive biology. The E. coli-based PURE (protein …
GN Kerestesy, KK Dods, CAL McFeely… - ACS Synthetic …, 2024 - ACS Publications
The tolerance of the translation apparatus toward noncanonical amino acids (ncAAs) has enabled the creation of diverse natural-product-like peptide libraries using mRNA display for …
A Danchin - Microbial Biotechnology, 2022 - Wiley Online Library
Living systems are studied using three complementary approaches: living cells, cell‐free systems and computer‐mediated modelling. Progresses in understanding, allowing …
Is it possible to build life? More specifically, is it possible to create a living synthetic cell from inanimate building blocks? This question precipitated into one of the most significant grand …