Designing and executing prime editing experiments in mammalian cells
Prime editing (PE) is a precision gene editing technology that enables the programmable
installation of substitutions, insertions and deletions in cells and animals without requiring …
installation of substitutions, insertions and deletions in cells and animals without requiring …
Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
Predicting prime editing efficiency and product purity by deep learning
Prime editing is a versatile genome editing tool but requires experimental optimization of the
prime editing guide RNA (pegRNA) to achieve high editing efficiency. Here we conducted a …
prime editing guide RNA (pegRNA) to achieve high editing efficiency. Here we conducted a …
[HTML][HTML] Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus
DY Kim, JM Lee, SB Moon, HJ Chin, S Park… - Nature …, 2022 - nature.com
Gene therapy would benefit from a miniature CRISPR system that fits into the small adeno-
associated virus (AAV) genome and has high cleavage activity and specificity in eukaryotic …
associated virus (AAV) genome and has high cleavage activity and specificity in eukaryotic …
Sequence determinant of small RNA production by DICER
YY Lee, H Kim, VN Kim - Nature, 2023 - nature.com
RNA silencing relies on specific and efficient processing of double-stranded RNA by Dicer,
which yields microRNAs (miRNAs) and small interfering RNAs (siRNAs),. However, our …
which yields microRNAs (miRNAs) and small interfering RNAs (siRNAs),. However, our …
[HTML][HTML] Precise transcript targeting by CRISPR-Csm complexes
D Colognori, M Trinidad, JA Doudna - Nature biotechnology, 2023 - nature.com
Robust and precise transcript targeting in mammalian cells remains a difficult challenge
using existing approaches due to inefficiency, imprecision and subcellular …
using existing approaches due to inefficiency, imprecision and subcellular …
[HTML][HTML] sgRNA sequence motifs blocking efficient CRISPR/Cas9-mediated gene editing
Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene
editing. High CRISPR/Cas9-mediated gene knockout efficiencies are essential for genetic …
editing. High CRISPR/Cas9-mediated gene knockout efficiencies are essential for genetic …
[HTML][HTML] PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Background The rapid expansion of the CRISPR toolbox through tagging effector domains
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
[HTML][HTML] Exonuclease-enhanced prime editors
DJJ Truong, J Geilenkeuser, SV Wendel… - Nature …, 2024 - nature.com
Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated
reverse transcription and integration of the de novo synthesized single-stranded DNA. To …
reverse transcription and integration of the de novo synthesized single-stranded DNA. To …
[HTML][HTML] Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance
The recent dramatic appearance of variants of concern of SARS-coronavirus-2 (SARS-CoV-
2) highlights the need for innovative approaches that simultaneously suppress viral …
2) highlights the need for innovative approaches that simultaneously suppress viral …