[HTML][HTML] It is time to crowd your cell culture media–physicochemical considerations with biological consequences
D Tsiapalis, DI Zeugolis - Biomaterials, 2021 - Elsevier
In vivo, the interior and exterior of cells is populated by various macromolecules that create
an extremely crowded milieu. Yet again, in vitro eukaryotic cell culture is conducted in dilute …
an extremely crowded milieu. Yet again, in vitro eukaryotic cell culture is conducted in dilute …
Bioinspired in vitro microenvironments to control cell fate: focus on macromolecular crowding
DI Zeugolis - American Journal of Physiology-Cell …, 2021 - journals.physiology.org
The development of therapeutic regenerative medicine and accurate drug discovery cell-
based products requires effective, with respect to obtaining sufficient numbers of viable …
based products requires effective, with respect to obtaining sufficient numbers of viable …
Factor defining the effects of tetraalkylammonium chloride on stability, folding, and dynamics of horse cytochrome c
This article describes the molecular mechanism by which tetraalkylammonium chloride ([R 4
N] Cl: R-= methyl (Me), ethyl (Et), propyl (Pr), butyl (Bu)) modulates the stability, folding, and …
N] Cl: R-= methyl (Me), ethyl (Et), propyl (Pr), butyl (Bu)) modulates the stability, folding, and …
Determinants for 1-alkyl-3-methylimidazolium chloride-induced modulation of thermodynamic stability and CO-association dynamics of horse ferrocytochrome c
This manuscript determines the factors by which the water-miscible ionic liquid (IL), 1-alkyl-3-
methylimidazolium chloride [Rmim] Cl affects the thermodynamic stability and CO …
methylimidazolium chloride [Rmim] Cl affects the thermodynamic stability and CO …
Role of macromolecular crowding on stability and iron release kinetics of serum transferrin
The macromolecular crowding influences the structural stability and functional properties of
transferrin (Tf). The equilibrium as well as kinetic studies of Tf at different concentrations of …
transferrin (Tf). The equilibrium as well as kinetic studies of Tf at different concentrations of …
Macromolecular crowding-induced molten globule states of the alkali pH-denatured proteins
Structural and molecular properties extracted from circular dichroism (CD), tryptophan
fluorescence and 1-anilino-8-napthalene sulfonate (ANS) binding experiments suggest that …
fluorescence and 1-anilino-8-napthalene sulfonate (ANS) binding experiments suggest that …
Analysis of the effect of 1-Allyl-3-Methylimidazolium chloride on thermodynamic stability, folding kinetics, and motional dynamics of horse cytochrome c
allyl-3-methylimidazolium chloride (AMIMCl) acts as a potential green solvent for proteins.
The present work provides a possible pathway by which the structural, kinetic …
The present work provides a possible pathway by which the structural, kinetic …
Factors defining the effects of macromolecular crowding on dynamics and thermodynamic stability of heme proteins in-vitro
The role of crowding agents on structure and activities of heme proteins has been
established. Analysis of kinetic and thermodynamic parameters measured for CO …
established. Analysis of kinetic and thermodynamic parameters measured for CO …
Determinants for macromolecular crowding-induced thermodynamic stabilization of acid-denatured cytochrome c to molten globules
The macromolecular crowding effect transforms the acid-denatured ferricytochrome c (cyt c
III)(U A-state) to molten-globule (MG MC-state) at pH 1.85. Crowding-induced stabilization …
III)(U A-state) to molten-globule (MG MC-state) at pH 1.85. Crowding-induced stabilization …
Effect of imidazolium based ionic liquids on CO-association dynamics and thermodynamic stability of Ferrocytochrome c
Abstract Analysis of kinetic and thermodynamic parameters measured for CO-association
reaction of Ferrocytochrome c (Ferrocyt c) under variable concentrations of 1-butyl-3 …
reaction of Ferrocytochrome c (Ferrocyt c) under variable concentrations of 1-butyl-3 …