Vertebrate vision critically depends on an 11-cis-retinoid renewal system known as the
visual cycle. At the heart of this metabolic pathway is an enzyme known as retinal pigment …

MicroRNAs are short, evolutionarily conserved noncoding RNAs that are critical for the
control of normal cellular physiology. In the retina, photoreceptors are highly specialized …

Delivering ribonucleoproteins (RNPs) for in vivo genome editing is safer than using viruses
encoding for Cas9 and its respective guide RNA. However, transient RNP activity does not …

In daylight, demand for visual chromophore (11-cis-retinal) exceeds supply by the classical
visual cycle. This shortfall is compensated, in part, by the retinal G-protein-coupled receptor …

Age-related macular degeneration (AMD) is one of the leading causes of vision loss in the
elderly. This disease involves oxidative stress burden in the retina leading to death of retinal …

Gene knockout is a technique routinely used in basic experimental research, particularly in
mouse skeletal and developmental studies. Tamoxifen-induced Cre/loxp system is known …

Background Cones are essential for color recognition, high resolution, and central vision;
therefore cone death causes blindness. Understanding the pathophysiology of each cell …

The retinal pigment epithelium (RPE) is an important monolayer of cells present in the outer
retina, forming a major part of the blood–retina barrier (BRB). It performs many tasks …

Retinal degenerative diseases such as age-related macular degeneration (AMD) represent
a leading cause of blindness, resulting in permanent damage to retinal cells that are …

Sterol homeostasis in mammalian cells and tissues involves balancing three fundamental
processes: de novo sterol biosynthesis; sterol import (eg, from blood-borne lipoproteins); …