Yeast expression systems: overview and recent advances

R Baghban, S Farajnia, M Rajabibazl, Y Ghasemi… - Molecular …, 2019 - Springer
Yeasts are outstanding hosts for the production of functional recombinant proteins with
industrial or medical applications. Great attention has been emerged on yeast due to the …

Recombinant protein production in yeasts

D Mattanovich, P Branduardi, L Dato, B Gasser… - Recombinant gene …, 2012 - Springer
Recombinant protein production is a multibillion-dollar market. The development of a new
product begins with the choice of a production host. While one single perfect host for every …

Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications

P Fickers, PH Benetti, Y Waché, A Marty… - FEMS yeast …, 2005 - academic.oup.com
The alkane-assimilating yeast Yarrowia lipolytica degrades very efficiently hydrophobic
substrates such as n-alkanes, fatty acids, fats and oils for which it has specific metabolic …

Biotechnological applications of Yarrowia lipolytica: past, present and future

HH Liu, XJ Ji, H Huang - Biotechnology Advances, 2015 - Elsevier
Non-conventional yeasts have attracted increasing interest due to their biochemical
characteristics and potential applications. Yarrowia lipolytica is a non-conventional yeast …

Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach

J Blazeck, L Liu, H Redden, H Alper - Applied and environmental …, 2011 - Am Soc Microbiol
The development of strong and tunable promoter elements is necessary to enable metabolic
and pathway engineering applications for any host organism. Here, we have expanded and …

Synthetic biology and molecular genetics in non-conventional yeasts: current tools and future advances

JM Wagner, HS Alper - Fungal Genetics and Biology, 2016 - Elsevier
Coupling the tools of synthetic biology with traditional molecular genetic techniques can
enable the rapid prototyping and optimization of yeast strains. While the era of yeast …

Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review

C Madzak, C Gaillardin, JM Beckerich - Journal of biotechnology, 2004 - Elsevier
The production of heterologous proteins is a research field of high interest, with both
academic and commercial applications. Yeasts offer a number of advantages as host …

Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering

C Madzak - Applied microbiology and biotechnology, 2015 - Springer
The oleaginous yeast Yarrowia lipolytica has become a recognized system for
expression/secretion of heterologous proteins. This non-conventional yeast is currently …

[PDF][PDF] Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica

C Madzak, B Tréton, S Blanchin-Roland - Journal of molecular …, 2000 - caister.com
The industrial yeast Yarrowia lipolytica secretes high amounts of an alkaline extracellular
protease encoded by the XPR2 gene. The industrial use of the XPR2 promoter was however …

Protein expression and secretion in the yeast Yarrowia lipolytica

JM Nicaud, C Madzak, P van den Broek… - FEMS yeast …, 2002 - academic.oup.com
Strains and vectors for protein expression and secretion have been developed in the yeast
Yarrowia lipolytica. Host strains were constructed with non-reverting auxotrophic markers …