CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a
technology that research scientists use to selectively modify the DNA of living organisms …

Highlights•Small molecules can tip the balance in favor of the HDR pathway over the NHEJ
pathway.•Cell cycle inhibitors and HDAC inhibitors can indirectly enhance precise …

Poria cocos is an important edible and medicinal fungus with a long history. However, the
lack of adequate genetic tools has hindered molecular genetic research and the genetic …

Cancer is the second leading cause of death globally and remains a major economic and
social burden. Although our understanding of cancer at the molecular level continues to …

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease
characterized by the degeneration of motor neurons. Mutations in the cyclin F (CCNF) and …

Simple Summary The production traits of pigs are generally regulated by multiple candidate
genes, which were associated with phenotype through genetic association analysis. In order …

Modeling a human disease is an essential part of biomedical research. The recent advances
in the field of molecular genetics made it possible to obtain genetically modified animals for …

Simple Summary Clustered regularly interspaced short palindromic repeats (CRISPR)
ribonucleoprotein (RNP)-mediated precise genome editing can modify a specific targeted …

Abstract Background Porcine fetal fibroblasts (PFFs) are important donor cells for generating
genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory …

The CRISPR/Cas technology of targeted genome editing made it possible to carry out
genetic engineering manipulations with eukaryotic genomes with a high efficiency. Targeted …