Much of the biopharmaceutical industry's success over the past 30 years has relied on
products derived from Chinese Hamster Ovary (CHO) cell lines. During this time …

As indicated by an ever-increasing number of FDA approvals, biotherapeutics constitute
powerful tools for the treatment of various diseases, with monoclonal antibodies (mAbs) …

Process intensification in mammalian cell culture-based recombinant protein production has
been achieved by high cell density perfusion exceeding 10 8 cells/mL in the recent years. As …

Human embryonic kidney cells HEK293 can be used for the production of therapeutic
glycoproteins requiring human post-translational modifications. High cell density perfusion …

Medium perfusion is critical in maintaining high cell concentration in cultures. The
conventional membrane filtration method for medium exchange has been challenged by the …

Over twenty-five years ago, perfusion was applied to mammalian cells to produce
therapeutic proteins, mainly sensitive ones. Nowadays, other issues such as process …

Chinese hamster ovary (CHO) cells are widely used to produce complex
biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand …

An 8 ton per year manufacturing facility is described based on the framework for integrated
and continuous bioprocessing (ICB) common to all known biopharmaceutical …

Transfusion of donor‐derived red blood cells (RBCs) is the most common form of cell
therapy. Production of transfusion‐ready cultured RBCs (cRBCs) is a promising replacement …

Understanding how mechanical stress affects erythropoiesis is crucial to produce
transfusable erythrocytes in fluid-turbulent bioreactors. We investigated the effects of shear …