Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

Despite significant progress, high-speed live-cell super-resolution studies remain limited to
specialized optical setups, generally requiring intense phototoxic illumination. Here, we …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision
profiling in a wide range of biomedical applications. However, current SR methods require …

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming
the diffraction limit in all three spatial dimensions by computing higher-order cumulants of …

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and
subcellular structures. However, going 'beyond the diffraction barrier'comes at a price, since …

High-density analysis methods for localization microscopy increase acquisition speed but
produce artifacts. We demonstrate that these artifacts can be eliminated by the combination …

Highlights•Spectral and biochemical diversity of GFP-like proteins continues to
increase.•GFP-unrelated fluorescent proteins open new areas of applications.•Red …

Correlative light and electron cryo-microscopy (cryo-CLEM) combines information from the
specific labeling of fluorescence cryo-microscopy (cryo-FM) with the high resolution in …