Highlights•The principles of protein structural ensemble determination are described.•The
use of experimental data averaged over multiple states is analysed.•Approaches for …

Highlights•Template based protein complex modeling has gone beyond homology
modeling.•Protein docking methods have improved upon their sampling/scoring …

Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during
cell division. An error correction mechanism drives the turnover of kinetochore-microtubule …

Accurate segregation of chromosomes relies on the force-bearing capabilities of the
kinetochore to robustly attach chromosomes to dynamic microtubule tips. The human Ska …

Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule
depolymerization to pull chromosomes apart during anaphase while resisting detachment …

DNA methylation patterns regulate gene expression programs and are maintained through a
highly coordinated process orchestrated by the RING E3 ubiquitin ligase UHRF1. UHRF1 …

Detailed mechanistic understanding of protein complex function is greatly enhanced by
insights from its 3-dimensional structure. Traditional methods of protein structure elucidation …

Many cancers overexpress one or more of the six human pro-survival BCL2 family proteins
to evade apoptosis. To determine which BCL2 protein or proteins block apoptosis in different …

The spindle assembly checkpoint kinase Mps1 not only inhibits anaphase but also corrects
erroneous attachments that could lead to missegregation and aneuploidy. However, Mps1's …

Accurate and precise structural ensembles of proteins and macromolecular complexes can
be obtained with metainference, a recently proposed Bayesian inference method that …