Methods of single-molecule fluorescence spectroscopy and microscopy
WE Moerner, DP Fromm - Review of Scientific instruments, 2003 - pubs.aip.org
Optical spectroscopy at the ultimate limit of a single molecule has grown over the past dozen
years into a powerful technique for exploring the individual nanoscale behavior of molecules …
years into a powerful technique for exploring the individual nanoscale behavior of molecules …
Single-molecule fluorescence studies of protein folding and conformational dynamics
Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …
of molecules: They give access to the complete distribution of observables (rather than only …
Observation of a Power-Law Memory Kernel for Fluctuations<? format?> within a Single Protein Molecule
The fluctuation of the distance between a fluorescein-tyrosine pair within a single protein
complex was directly monitored in real time by photoinduced electron transfer and found to …
complex was directly monitored in real time by photoinduced electron transfer and found to …
Single-molecule michaelis− menten equations
This paper summarizes our present theoretical understanding of single-molecule kinetics
associated with the Michaelis− Menten mechanism of enzymatic reactions. Single-molecule …
associated with the Michaelis− Menten mechanism of enzymatic reactions. Single-molecule …
Multidimensional tunneling, recrossing, and the transmission coefficient for enzymatic reactions
Although many aspects of enzyme catalysis have been constructively analyzed, 1-52 there
are still many aspects that are imperfectly understood. Of particular interest in this regard are …
are still many aspects that are imperfectly understood. Of particular interest in this regard are …
Theory of single-molecule spectroscopy: beyond the ensemble average
▪ Abstract Single-molecule spectroscopy (SMS) is a powerful experimental technique used
to investigate a wide range of physical, chemical, and biophysical phenomena. The merit of …
to investigate a wide range of physical, chemical, and biophysical phenomena. The merit of …
Single-molecule optics
F Kulzer, M Orrit - Annu. Rev. Phys. Chem., 2004 - annualreviews.org
▪ Abstract We review recent developments in single-molecule spectroscopy and microscopy.
New optical methods provide access to the absorption, emission, or excitation spectra of …
New optical methods provide access to the absorption, emission, or excitation spectra of …
Stretched exponential decay and correlations in the catalytic activity of fluctuating single lipase molecules
O Flomenbom, K Velonia, D Loos… - Proceedings of the …, 2005 - National Acad Sciences
Single-molecule techniques offer a unique tool for studying the dynamical behavior of
individual molecules and provide the possibility to construct distributions from individual …
individual molecules and provide the possibility to construct distributions from individual …
Single-molecule approach to dispersed kinetics and dynamic disorder: Probing conformational fluctuation and enzymatic dynamics
X Sunney Xie - The Journal of chemical physics, 2002 - pubs.aip.org
This article reviews our efforts in understanding dynamical fluctuations of both conformation
and enzymatic reactivity in single biomolecules. The single-molecule approach is shown to …
and enzymatic reactivity in single biomolecules. The single-molecule approach is shown to …
Single-molecule enzymology: stochastic Michaelis–Menten kinetics
We provide a stochastic analysis of single-molecule enzymatic reactions that follow
Michaelis–Menten kinetics. We show that this system can exhibit oscillatory behavior in the …
Michaelis–Menten kinetics. We show that this system can exhibit oscillatory behavior in the …