Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …

Whenever gene expression is being examined, it is essential that a normalization process is
carried out to eliminate non-biological variations. The use of reference genes, such as …

Abstract The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-
causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic …

Quantitative real-time polymerase chain reaction (qRT-PCR) is a valuable tool for estimating
gene expression; however, the validity is largely dependent on the selection of stable …

Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is
a momentous tool for calculating the expression levels of targeted genes across various …

The green peach aphid, Myzus persicae Sulzer (Hemiptera, Aphididae), is an important
cosmopolitan pest. Real time qRT‐PCR has been used for target gene expression analysis …

Liriomyza trifolii is a highly-invasive leafmining insect that causes significant damage to
vegetables and horticultural crops worldwide. Relatively few studies have quantified gene …

Real-time quantitative PCR (qPCR) is a reliable and widely used technique for analyzing the
expression profiles of target genes in different species, and reference genes with stable …

The white-backed planthopper, Sogatella furcifera (Hemiptera, Delphacidae), is one of the
most devastating rice pests. For a better control strategy, various genetic studies have been …

The oriental armyworm, Mythimna separata, is a major insect pest in China and other Asian
countries. Unfortunately, suitable reference genes for quantitative real-time PCR (qRT-PCR) …