Platelet microparticles: detection and assessment of their paradoxical functional roles in disease and regenerative medicine

T Burnouf, HA Goubran, ML Chou, D Devos… - Blood reviews, 2014 - Elsevier
There is increasing research on and clinical interest in the physiological role played by
platelet microparticles (PMPs). PMPs are 0.1–1-μm fragments shed from plasma …

Measurement of microvesicle levels in human blood using flow cytometry

WL Chandler - Cytometry Part B: Clinical Cytometry, 2016 - Wiley Online Library
Microvesicles are fragments of cells released when the cells are activated, injured, or
apoptotic. Analysis of microvesicle levels in blood has the potential to shed new light on the …

Platelets confound the measurement of extracellular miRNA in archived plasma

AJ Mitchell, WD Gray, SS Hayek, YA Ko, S Thomas… - Scientific reports, 2016 - nature.com
Extracellular miRNAs are detectable in biofluids and represent a novel class of disease
biomarker. Although many studies have utilized archived plasma for miRNA biomarker …

Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women

RA Dragovic, JH Southcombe, DS Tannetta… - Biology of …, 2013 - academic.oup.com
Excessive release of syncytiotrophoblast extracellular vesicles (STBMs) from the placenta
into the maternal circulation may contribute to the systemic inflammation that is characteristic …

Characterisation of adipocyte-derived extracellular vesicles released pre-and post-adipogenesis

KD Connolly, IA Guschina, V Yeung… - Journal of …, 2015 - Taylor & Francis
Extracellular vesicles (EVs) are submicron vesicles released from many cell types, including
adipocytes. EVs are implicated in the pathogenesis of obesity-driven cardiovascular …

Diameters and fluorescence calibration for extracellular vesicle analyses by flow cytometry

P Simeone, C Celia, G Bologna, E Ercolino… - International Journal of …, 2020 - mdpi.com
Extracellular vesicles (EVs) play a crucial role in the intercellular crosstalk. Mesenchymal
stem cell-derived EVs (MSC-EVs), displaying promising therapeutic roles, contribute to the …

Flow cytometry analysis of circulating extracellular vesicle subtypes from fresh peripheral blood samples

M Marchisio, P Simeone, G Bologna, E Ercolino… - International journal of …, 2020 - mdpi.com
Extracellular vesicles (EVs) are released by shedding during different physiological
processes and are increasingly thought to be new potential biomarkers. However, the …

Tips and tricks for flow cytometry-based analysis and counting of microparticles

P Poncelet, S Robert, N Bailly, F Garnache-Ottou… - … and Apheresis Science, 2015 - Elsevier
Submicron-sized extra-cellular vesicles generated by budding from the external cell
membranes, microparticles (MPs) are important actors in transfusion as well as in other …

Role of extracellular vesicles in pulmonary arterial hypertension: modulation of pulmonary endothelial function and angiogenesis

A Khandagale, M Åberg, G Wikström… - … , and vascular biology, 2020 - Am Heart Assoc
Objective: Extracellular vesicles (EVs) have the potential to act as intercellular
communicators. The aims were to characterize circulating EVs in patients with pulmonary …

Elevated circulating VE-cadherin+ CD144+ endothelial microparticles in ischemic cerebrovascular disease

P Li, C Qin - Thrombosis research, 2015 - Elsevier
Objectives Circulating endothelial microparticles act as biological markers of endothelial
function that reflect vascular injury. Here, we examined the hypothesis that the quantity of …