Advances and opportunities in single-cell transcriptomics for plant research

C Seyfferth, J Renema, JR Wendrich… - Annual Review of …, 2021 - annualreviews.org
Single-cell approaches are quickly changing our view on biological systems by increasing
the spatiotemporal resolution of our analyses to the level of the individual cell. The field of …

Ten years of next-generation sequencing technology

EL Van Dijk, H Auger, Y Jaszczyszyn, C Thermes - Trends in genetics, 2014 - cell.com
Ten years ago next-generation sequencing (NGS) technologies appeared on the market.
During the past decade, tremendous progress has been made in terms of speed, read …

Multiplexed droplet single-cell RNA-sequencing using natural genetic variation

HM Kang, M Subramaniam, S Targ, M Nguyen… - Nature …, 2018 - nature.com
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel
profiling of transcriptomes. However, assessing differential expression across multiple …

Single-cell barcoding and sequencing using droplet microfluidics

R Zilionis, J Nainys, A Veres, V Savova, D Zemmour… - Nature protocols, 2017 - nature.com
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

[HTML][HTML] Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells

AM Klein, L Mazutis, I Akartuna, N Tallapragada… - Cell, 2015 - cell.com
It has long been the dream of biologists to map gene expression at the single-cell level. With
such data one might track heterogeneous cell sub-populations, and infer regulatory …

Power analysis of single-cell RNA-sequencing experiments

V Svensson, KN Natarajan, LH Ly, RJ Miragaia… - Nature …, 2017 - nature.com
Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method
to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and …

[HTML][HTML] Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems

X Zhang, T Li, F Liu, Y Chen, J Yao, Z Li, Y Huang… - Molecular cell, 2019 - cell.com
Since its establishment in 2009, single-cell RNA sequencing (RNA-seq) has been a major
driver behind progress in biomedical research. In developmental biology and stem cell …

Single-cell RNA-seq: advances and future challenges

AE Saliba, AJ Westermann, SA Gorski… - Nucleic acids …, 2014 - academic.oup.com
Phenotypically identical cells can dramatically vary with respect to behavior during their
lifespan and this variation is reflected in their molecular composition such as the …

[HTML][HTML] Small but perfectly formed? Successes, challenges, and opportunities for microfluidics in the chemical and biological sciences

DT Chiu, AJ Demello, D Di Carlo, PS Doyle, C Hansen… - Chem, 2017 - cell.com
Microfluidic systems are pervasive in many areas of experimental science, but what are the
real advantages of this technology? We describe some of the features and properties that …

[HTML][HTML] The transcriptome and DNA methylome landscapes of human primordial germ cells

F Guo, L Yan, H Guo, L Li, B Hu, Y Zhao, J Yong, Y Hu… - Cell, 2015 - cell.com
Germ cells are vital for transmitting genetic information from one generation to the next and
for maintaining the continuation of species. Here, we analyze the transcriptome of human …