Solid-state NMR investigations of extracellular matrixes and cell walls of algae, bacteria, fungi, and plants

N Ghassemi, A Poulhazan, F Deligey… - Chemical …, 2021 - ACS Publications
Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for
supporting cell integrity and function and regulating intercellular communication. These …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR

A Chakraborty, LD Fernando, W Fang… - Nature …, 2021 - nature.com
Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall,
but the supramolecular architecture of this carbohydrate-rich composite remains …

Structural polymorphism of chitin and chitosan in fungal cell walls from solid-state NMR and principal component analysis

LD Fernando, MC Dickwella Widanage… - Frontiers in Molecular …, 2021 - frontiersin.org
Chitin is a major carbohydrate component of the fungal cell wall and a promising target for
novel antifungal agents. However, it is technically challenging to characterize the structure of …

Adaptative survival of Aspergillus fumigatus to echinocandins arises from cell wall remodeling beyond β− 1, 3-glucan synthesis inhibition

MC Dickwella Widanage, I Gautam, D Sarkar… - Nature …, 2024 - nature.com
Antifungal echinocandins inhibit the biosynthesis of β− 1, 3-glucan, a major and essential
polysaccharide component of the fungal cell wall. However, the efficacy of echinocandins …

Solid-state NMR of plant and fungal cell walls: a critical review

W Zhao, LD Fernando, A Kirui, F Deligey… - Solid state nuclear …, 2020 - Elsevier
The cell walls of plants and microbes are a central source for bio-renewable energy and the
major targets of antibiotics and antifungal agents. It is highly challenging to determine the …

Structure of In Vitro-Synthesized Cellulose Fibrils Viewed by Cryo-Electron Tomography and 13C Natural-Abundance Dynamic Nuclear Polarization Solid-State …

F Deligey, MA Frank, SH Cho, A Kirui… - …, 2022 - ACS Publications
Cellulose, the most abundant biopolymer, is a central source for renewable energy and
functionalized materials. In vitro synthesis of cellulose microfibrils (CMFs) has become …

Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues

JP Tingley, KE Low, X Xing, DW Abbott - Biotechnology for biofuels, 2021 - Springer
The production of biofuels as an efficient source of renewable energy has received
considerable attention due to increasing energy demands and regulatory incentives to …

Polysaccharide assemblies in fungal and plant cell walls explored by solid-state NMR

LD Fernando, W Zhao, I Gautam, A Ankur, T Wang - Structure, 2023 - cell.com
Structural analysis of macromolecular complexes within their natural cellular environment
presents a significant challenge. Recent applications of solid-state NMR (ssNMR) …

[HTML][HTML] Covalent connectivity of glycogen in brewer's spent yeast cell walls revealed by enzymatic approaches and dynamic nuclear polarization NMR

R Bastos, I Marín-Montesinos, SS Ferreira… - Carbohydrate …, 2024 - Elsevier
Yeast cell walls undergo modifications during the brewing process, leading to a remodelling
of their architecture. One significant change is the increased insolubility of the cell wall …