Metabolic engineering allows development of microbial strains efficiently producing
chemicals and materials, but it requires much time, effort, and cost to make the strains …

DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently
been developed. However, especially when multiple genetic manipulations are required …

The mTORC1 (mechanistic target of rapamycin complex 1) protein kinase regulates growth
in response to nutrients and growth factors. Nutrients promote its translocation to the …

The combination of synthetic and systems biology is a powerful framework to study
fundamental questions in biology and produce chemicals of immediate practical application …

Gene expression control based on CRISPRi (clustered regularly interspaced short
palindromic repeats interference) has emerged as a powerful tool for creating synthetic gene …

Natural products (NPs) are critical sources of drug molecules for decades. About two-thirds
of natural antibiotics are produced by Streptomyces. Streptomyces have a large number of …

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-
dependent cloning methods for reasons of efficiency and performance. Here, we describe …

The rise of antibiotic resistance demands the acceleration of molecular diversification
strategies to inspire new chemical entities for antibiotic medicines. We report here on the …