Engineering of thermostable enzymes for industrial applications

F Rigoldi, S Donini, A Redaelli, E Parisini… - APL …, 2018 - pubs.aip.org
The catalytic properties of some selected enzymes have long been exploited to carry out
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …

SARS-CoV-2 variant prediction and antiviral drug design are enabled by RBD in vitro evolution

J Zahradník, S Marciano, M Shemesh, E Zoler… - Nature …, 2021 - nature.com
SARS-CoV-2 variants of interest and concern will continue to emerge for the duration of the
COVID-19 pandemic. To map mutations in the receptor-binding domain (RBD) of the spike …

High‐throughput droplet‐based microfluidics for directed evolution of enzymes

FWY Chiu, S Stavrakis - Electrophoresis, 2019 - Wiley Online Library
Natural enzymes have evolved over millions of years to allow for their effective operation
within specific environments. However, it is significant to note that despite their wide …

SARS-CoV-2 RBD in vitro evolution follows contagious mutation spread, yet generates an able infection inhibitor

J Zahradník, S Marciano, M Shemesh, E Zoler… - BioRxiv, 2021 - biorxiv.org
SARS-CoV-2 is continually evolving, with more contagious mutations spreading rapidly.
Using in vitro evolution to affinity maturate the receptor-binding domain (RBD) of the spike …

A genetic switch controls Pseudomonas aeruginosa surface colonization

C Manner, R Dias Teixeira, D Saha… - Nature …, 2023 - nature.com
Efficient colonization of mucosal surfaces is essential for opportunistic pathogens like
Pseudomonas aeruginosa, but how bacteria collectively and individually adapt to optimize …

[图书][B] Encyclopedia of Supramolecular Chemistry-Two-Volume Set (Print)

JL Atwood - 2013 - books.google.com
The two-volume Encyclopedia of Supramolecular Chemistry offers authoritative, centralized
information on a rapidly expanding interdisciplinary field. User-friendly and high-quality …

Random mutagenesis by error-prone PCR

EO McCullum, BAR Williams, J Zhang… - In Vitro Mutagenesis …, 2010 - Springer
In vitro selection coupled with directed evolution represents a powerful method for
generating nucleic acids and proteins with desired functional properties. Creating high …

The Mother Enrichment Program: A Genetic System for Facile Replicative Life Span Analysis in Saccharomyces cerevisiae

DL Lindstrom, DE Gottschling - Genetics, 2009 - academic.oup.com
The replicative life span (RLS) of Saccharomyces cerevisiae has been established as a
model for the genetic regulation of longevity despite the inherent difficulty of the RLS assay …

Programmable artificial cells using histamine-responsive synthetic riboswitch

M Dwidar, Y Seike, S Kobori, C Whitaker… - Journal of the …, 2019 - ACS Publications
Artificial cells that encapsulate DNA-programmable protein expression machinery are
emerging as an attractive platform for studying fundamental cellular properties and …

SoxR as a Single-Cell Biosensor for NADPH-Consuming Enzymes in Escherichia coli

S Siedler, G Schendzielorz, S Binder… - ACS synthetic …, 2014 - ACS Publications
An ultra-high-throughput screening system for NADPH-dependent enzymes, such as
stereospecific alcohol dehydrogenases, was established. It is based on the [2Fe–2S] cluster …