[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …
molecules have led to substantial improvements in accuracy, read length and throughput …
[HTML][HTML] Third-generation sequencing: the spearhead towards the radical transformation of modern genomics
K Athanasopoulou, MA Boti, PG Adamopoulos… - Life, 2021 - mdpi.com
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
[HTML][HTML] Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing
Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been
difficult in part because a simple method to simultaneously quantify their abundance and …
difficult in part because a simple method to simultaneously quantify their abundance and …
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore
PN Pratanwanich, F Yao, Y Chen, CWQ Koh… - Nature …, 2021 - nature.com
RNA modifications, such as N 6-methyladenosine (m6A), modulate functions of cellular RNA
species. However, quantifying differences in RNA modifications has been challenging. Here …
species. However, quantifying differences in RNA modifications has been challenging. Here …
[HTML][HTML] Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing
Abstract N6-methyladenosine (m6A) has been increasingly recognized as a new and
important regulator of gene expression. To date, transcriptome-wide m6A detection primarily …
important regulator of gene expression. To date, transcriptome-wide m6A detection primarily …
Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing
Nanopore RNA sequencing shows promise as a method for discriminating and identifying
different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing …
different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing …
[HTML][HTML] Detection of m6A from direct RNA sequencing using a multiple instance learning framework
RNA modifications such as m6A methylation form an additional layer of complexity in the
transcriptome. Nanopore direct RNA sequencing can capture this information in the raw …
transcriptome. Nanopore direct RNA sequencing can capture this information in the raw …
The RNA modification N6-methyladenosine as a novel regulator of the immune system
Z Shulman, N Stern-Ginossar - Nature immunology, 2020 - nature.com
Protection from harmful pathogens depends on activation of the immune system, which
relies on tight regulation of gene expression. Recently, the RNA modification N 6 …
relies on tight regulation of gene expression. Recently, the RNA modification N 6 …
DirectRMDB: a database of post-transcriptional RNA modifications unveiled from direct RNA sequencing technology
With advanced technologies to map RNA modifications, our understanding of them has
been revolutionized, and they are seen to be far more widespread and important than …
been revolutionized, and they are seen to be far more widespread and important than …
Genomics in the long-read sequencing era
EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …
explore genomes. While in the early years these methods suffered technical limitations, they …