Signal transduction network principles underlying bacterial collective behaviors
AA Bridges, JA Prentice, NS Wingreen… - Annual review of …, 2022 - annualreviews.org
Bacteria orchestrate collective behaviors and accomplish feats that would be unsuccessful if
carried out by a lone bacterium. Processes undertaken by groups of bacteria include …
carried out by a lone bacterium. Processes undertaken by groups of bacteria include …
Microbial single-cell RNA sequencing by split-pool barcoding
INTRODUCTION Bacteria heterogeneously activate gene expression programs in response
to environmental changes, stress, and other stimuli. Such behavior may serve as a bet …
to environmental changes, stress, and other stimuli. Such behavior may serve as a bet …
Microfluidics for long-term single-cell time-lapse microscopy: Advances and applications
Cells are inherently dynamic, whether they are responding to environmental conditions or
simply at equilibrium, with biomolecules constantly being made and destroyed. Due to their …
simply at equilibrium, with biomolecules constantly being made and destroyed. Due to their …
Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Large synthetic genetic circuits require the simultaneous expression of many regulators.
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
Escherichia coli can survive stress by noisy growth modulation
Gene expression can be noisy, as can the growth of single cells. Such cell-to-cell variation
has been implicated in survival strategies for bacterial populations. However, it remains …
has been implicated in survival strategies for bacterial populations. However, it remains …
Dynamic gene expression and growth underlie cell-to-cell heterogeneity in Escherichia coli stress response
NMV Sampaio, CM Blassick… - Proceedings of the …, 2022 - National Acad Sciences
Cell-to-cell heterogeneity in gene expression and growth can have critical functional
consequences, such as determining whether individual bacteria survive or die following …
consequences, such as determining whether individual bacteria survive or die following …
Isolating live cells after high-throughput, long-term, time-lapse microscopy
Single-cell genetic screens can be incredibly powerful, but current high-throughput platforms
do not track dynamic processes, and even for non-dynamic properties they struggle to …
do not track dynamic processes, and even for non-dynamic properties they struggle to …
Phage-encoded sigma factors alter bacterial dormancy
DA Schwartz, BK Lehmkuhl, JT Lennon - Msphere, 2022 - Am Soc Microbiol
By entering a reversible state of reduced metabolic activity, dormant microorganisms are
able to tolerate suboptimal conditions that would otherwise reduce their fitness. Dormancy …
able to tolerate suboptimal conditions that would otherwise reduce their fitness. Dormancy …
Stochastic antagonism between two proteins governs a bacterial cell fate switch
Cell fate decision circuits must be variable enough for genetically identical cells to adopt a
multitude of fates, yet ensure that these states are distinct, stably maintained, and …
multitude of fates, yet ensure that these states are distinct, stably maintained, and …
Bacterial vivisection: how fluorescence-based imaging techniques shed a light on the inner workings of bacteria
A Cambré, A Aertsen - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
The rise in fluorescence-based imaging techniques over the past 3 decades has improved
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …
the ability of researchers to scrutinize live cell biology at increased spatial and temporal …