Abstract The development of Ribosome Profiling (RiboSeq) has revolutionized functional
genomics. RiboSeq is based on capturing and sequencing of the mRNA fragments enclosed …

By mapping the positions of millions of translating ribosomes in the cell, ribosome profiling
(Ribo-seq) has established its role as a powerful tool to study gene expression. Several …

Single-cell sequencing methods have enabled in-depth analysis of the diversity of cell types
and cell states in a wide range of organisms. These tools focus predominantly on …

Translation is one of the fundamental processes of life. It comprises the assembly of
polypeptides whose amino acid sequence corresponds to the codon sequence of an …

Functional protein-coding small open reading frames (smORFs) are emerging as an
important class of genes. However, the number of translated smORFs in the human genome …

In eukaryotes, ribosome profiling provides insight into the mechanism of protein synthesis at
the codon level. In bacteria, however, the method has been more problematic and no …

Ribosome movement is not always smooth and is rather often impeded. For ribosome
pauses, fundamental issues remain to be addressed, including where ribosomes pause on …

Covalent chemical modifications of cellular RNAs directly impact all biological processes.
However, our mechanistic understanding of the enzymes catalyzing these modifications …

Translational reprogramming is a fundamental layer of immune regulation, but how such a
global regulatory mechanism operates remains largely unknown. Here we perform a genetic …

Protein synthesis represents a major metabolic activity of the cell. However, how it is affected
by aging and how this in turn impacts cell function remains largely unexplored. To address …