Soil sample sizes for DNA extraction substantially affect the examination of microbial diversity and co-occurrence patterns but not abundance

T Li, S Zhang, J Hu, H Hou, K Li, Q Fan, F Wang… - Soil Biology and …, 2023 - Elsevier
As the cornerstone of most soil microbial diversity and abundance research, soil DNA
extraction is inherently influenced by soil sample sizes, yet the effects remain far from fully …

Nucleic acid based testing (NABing): a game changer technology for public health

HK Khera, R Mishra - Molecular Biotechnology, 2024 - Springer
Timely and accurate detection of the causal agent of a disease is crucial to restrict suffering
and save lives. Mere symptoms are often not enough to detect the root cause of the disease …

Sampling and analysis of the maize microbiome

JG Wallace, A Favela, S Raglin - Cold Spring Harbor …, 2024 - cshprotocols.cshlp.org
Maize is an important plant for both global food security and genetics research. As the
importance of microorganisms to plant health is becoming clearer, there is a growing interest …

[HTML][HTML] Biochar dose determines methane uptake and methanotroph abundance in Haplic Luvisol

A Kubaczyński, A Walkiewicz, A Pytlak… - Science of The Total …, 2022 - Elsevier
Biochar promotes C sequestration and improvement of soil properties. Nevertheless, the
effects of biochar addition on soil condition are poorly understood, especially with respect to …

Optimised protocol for the extraction of fish DNA from freshwater sediments

G Thomson‐Laing, JD Howarth… - Freshwater …, 2022 - Wiley Online Library
Monitoring fish is necessary for understanding population dynamics, tracking distribution
patterns and evaluating conservation efforts. Molecular techniques targeting environmental …

Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)

MK Nwe, N Jangpromma, L Taemaitree - Scientific Reports, 2024 - nature.com
Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform
assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is …

Assessing benthic macroinvertebrate communities' spatial heterogeneity in Mediterranean transitional waters through eDNA metabarcoding

M Pinna, F Zangaro, V Specchia - Scientific Reports, 2024 - nature.com
Transitional waters are important habitats both for biodiversity and ecological functions,
providing valuable natural resources and relevant ecosystem services. However, they are …

Tracing early pastoralism in Central Europe using sedimentary ancient DNA

G Zampirolo, LE Holman, R Sawafuji, M Ptáková… - Current Biology, 2024 - cell.com
Central European forests have been shaped by complex human interactions throughout the
Holocene, with significant changes following the introduction of domesticated animals in the …

[HTML][HTML] Ascomycota and Basidiomycota fungal phyla as indicators of land use efficiency for soil organic carbon accrual with woody plantations

LM Manici, F Caputo, F Fornasier, A Paletto, E Ceotto… - Ecological …, 2024 - Elsevier
As soil fungi are major players in the carbon accumulation process, the two main fungal
degraders in topsoil, Ascomycetes and Basidiomycetes, were investigated as indicators of …

Improved method for the extraction of high-quality DNA from lignocellulosic compost samples for metagenomic studies

ÂMA Costa, AO Santos, J Sousa, JL Rodrigues… - Applied Microbiology …, 2021 - Springer
The world economy is currently moving towards more sustainable approaches.
Lignocellulosic biomass has been widely used as a substitute for fossil sources since it is …