Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA
fragments, is a powerful tool for globally monitoring translation in vivo. The method has …

The translation of messenger RNA (mRNA) into protein and the folding of the resulting
protein into an active form are prerequisites for virtually every cellular process and represent …

Ribosome profiling has emerged as a technique for measuring translation comprehensively
and quantitatively by deep sequencing of ribosome-protected mRNA fragments. By …

Genome-wide analyses of gene expression have so far focused on the abundance of mRNA
species as measured either by microarray or, more recently, by RNA sequencing. However …

RNA-sequencing protocols can quantify gene expression regulation from transcription to
protein synthesis. Ribosome profiling (Ribo-seq) maps the positions of translating ribosomes …

Synonymous mutations change the sequence of a gene without directly altering the
sequence of the encoded protein. Here, we present evidence that these" silent" mutations …

Short open reading frames (sORFs) are a common feature of all genomes, but their coding
potential has mostly been disregarded, partly because of the difficulty in determining …

The human transcriptome comprises> 80000 protein-coding transcripts and the estimated
number of proteins synthesized from these transcripts is in the range of 250000 to 1 million …

Gene evolution has long been thought to be primarily driven by duplication and
rearrangement mechanisms. However, every evolutionary lineage harbours orphan genes …

Stop codon readthrough (SCR) occurs when the ribosome miscodes at a stop codon. Such
readthrough events can be therapeutically desirable when a premature termination codon …