Multiplex immunofluorescence is a powerful tool for the simultaneous detection of tissue‐
based biomarkers, revolutionising traditional immunohistochemistry. The Opal methodology …

Background Colorectal cancer is one of the most frequent and deadly tumors. Among the
key regulators of CRC growth and progression, the microenvironment has emerged as a …

Immune responses contribute to tissue injury and repair during and after ischemic stroke.
However, the spatiotemporal and initiating molecular events remain incompletely …

Immune checkpoint inhibitor-associated colitis (ICIC) affects approximately 15% of cancer
patients treated with immunotherapy. Although histological evaluation is potentially valuable …

Colorectal cancer (CRC) remains one of the deadliest malignancies worldwide despite
recent progress in treatment strategies. Though immune checkpoint inhibition has proven …

Inflammatory bowel disease (IBD) is characterized by chronic, dysregulated inflammation in
the gastrointestinal tract. The heterogeneity of IBD is reflected through two major subtypes …

As our understanding of the tumor microenvironment grows, the pathology field is
increasingly utilizing multianalyte diagnostic assays to understand important characteristics …

Multiplex immunohistochemistry (mIHC) enables simultaneous staining of multiple immune
markers on a single tissue section. Mounting studies have demonstrated the versatility of …

Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various
immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC …

The past few years have seen remarkable success in the field of oncology with the advent of
cancer immunotherapies and checkpoint inhibitors. The effectiveness of immunomodulatory …