Integrated approaches using different in vitro methods in combination with bioinformatics
can (i) increase the success rate and speed of drug development;(ii) improve the accuracy of …

In general, herbal medicines have been considered as safe by the general public, since they
are naturally occurring and have been applied in treatment for over thousands of years. As …

Drug-induced human hepatotoxicity is difficult to predict using the current in vitro systems. In
this study, long-term 3D organotypic cultures of the human hepatoma HepaRG cell line were …

Existing approaches to evaluate cell viability involve cell staining with chemical reagents.
However, the step of exogenous staining makes these methods undesirable for rapid …

Predictive in vitro models alternative to in vivo animal will have a significant impact in
toxicology. Conventional 2D models do not reflect the complexity of a 3D organ resulting in …

As the fields of tissue engineering and regenerative medicine mature toward clinical
applications, the need for online monitoring both for quantitative and qualitative use …

Decellularized organ scaffolds allow whole organ regeneration and study of cell behavior in
three-dimensional culture conditions. Cell viability within the bio-engineered organ …

Bone tissue engineering (TE) aims to develop reproducible and predictive three-
dimensional (3D) TE constructs, defined as cell-seeded scaffolds produced by a controlled …

In vivo studies clearly demonstrate the participation and subsequent death of non-
parenchymal liver cells (NPCs) with corresponding hepatocyte effects. This results in a …

The determination of metabolic fluxes provides detailed information of cellular physiology,
and the assessment of metabolic flux changes upon a certain perturbation can help to …