New Developments in PCR-Based Diagnostics for Bacterial Pathogens Causing Gastrointestinal Infections—A Narrative Mini-Review on Challenges in the Tropics

U Loderstädt, RM Hagen, A Hahn… - Tropical Medicine and …, 2021 - mdpi.com
The application of modern PCR approaches for the diagnosis of bacterial gastrointestinal
pathogens is on the rise due to their rapidly available results combined with high sensitivity …

Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of Cryptosporidium …

F Weinreich, A Hahn, KA Eberhardt, T Feldt, FS Sarfo… - Pathogens, 2021 - mdpi.com
As qualified microscopy of enteric parasitoses as defined by high diagnostic accuracy is
difficult to maintain in non-endemic areas due to scarce opportunities for practicing with …

Study of enteric pathogens among children in the tropics and effects of prolonged storage of stool samples

U Schotte, T Hoffmann, NG Schwarz… - Letters in Applied …, 2021 - academic.oup.com
The study was performed to compare real‐time PCR after nucleic acid extraction directly
from stool samples as well as from samples stored and transported on Whatman papers or …

Comparison of three filter paper-based devices for safety and stability of viral sample collection in poultry

S Wannaratana, A Thontiravong, S Pakpinyo - Avian Pathology, 2021 - Taylor & Francis
General diagnosis of poultry viruses primarily relies on detection of viruses in samples, but
many farms are located in remote areas requiring logistic transportation. Filter paper cards …

Long-term preservation and storage of faecal samples in whatman® cards for pcr detection and genotyping of giardia duodenalis and cryptosporidium hominis

PC Köster, B Bailo, A Dashti, C Hernández-Castro… - Animals, 2021 - mdpi.com
Simple Summary Preservation and storage of biological samples prior to testing and
analysis is a pressing issue in the epidemiological field studies conducted in remote or poor …

A new solid matrix for preservation of viral nucleic acid from clinical specimens at ambient temperature

T Cromeans, N Jothikumar, J Lee, N Collins… - Journal of virological …, 2019 - Elsevier
Stabilizing paper matrix methods for retaining nucleic acid from inactivated clinical
specimens offer a solution for molecular diagnostics when specimens may be stored or …

Use of Chelex-100 for the molecular diagnosis of five animal pathogens

ML Tomazic, M Hamer, CP Bustos… - Revista FAVE …, 2021 - SciELO Argentina
Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is
a key step for downstream applications. This work aimed to compare the DNA extraction …

[PDF][PDF] FACULTAD DE CIENCIAS E INGENIERÍA

MAL Mendoza - 2024 - repositorio.upch.edu.pe
La enfermedad diarreica es una de las causas principales de muerte en niños menores de
cinco años. Uno de los patógenos involucrados en esta enfermedad especialmente en …