Application‐based guidelines for best practices in plant flow cytometry

E Sliwinska, J Loureiro, IJ Leitch, P Šmarda… - Cytometry Part …, 2022 - Wiley Online Library
Flow cytometry (FCM) is currently the most widely‐used method to establish nuclear DNA
content in plants. Since simple, 1‐3‐parameter, flow cytometers, which are sufficient for most …

Isolation of plant nuclei for estimation of nuclear DNA content: overview and best practices

J Loureiro, P Kron, EM Temsch, P Koutecký… - Cytometry Part …, 2021 - Wiley Online Library
A critical aspect for obtaining accurate, reliable, and high‐resolution estimates of nuclear
DNA content is the release of nuclei from the cytoplasm in sufficient amounts, while …

The application of flow cytometry for estimating genome size, ploidy level endopolyploidy, and reproductive modes in plants

J Pellicer, RF Powell, IJ Leitch - Molecular plant taxonomy: methods and …, 2021 - Springer
Over the years, the amount of DNA in a nucleus (genome size) has been estimated using a
variety of methods, but increasingly, flow cytometry (FCM) has become the method of choice …

Correlations of polyploidy and apomixis with elevation and associated environmental gradients in an alpine plant

CCF Schinkel, B Kirchheimer, AS Dellinger, S Klatt… - AoB Plants, 2016 - academic.oup.com
Apomictic plants expand their geographical distributions more to higher elevations
compared to their sexual progenitors. It was so far unclear whether this tendency is related …

Pathways to polyploidy: indications of a female triploid bridge in the alpine species Ranunculus kuepferi (Ranunculaceae)

CCF Schinkel, B Kirchheimer, S Dullinger… - Plant Systematics and …, 2017 - Springer
Polyploidy is one of the most important evolutionary processes in plants. In natural
populations, polyploids usually emerge from unreduced gametes which either fuse with …

Emergence of apospory and bypass of meiosis via apomixis after sexual hybridisation and polyploidisation

D Hojsgaard, J Greilhuber, M Pellino, O Paun… - New …, 2014 - Wiley Online Library
Hybridisation and polyploidy are major forces contributing to plant speciation. Homoploid
(2x) and heteroploid (3x) hybrids, however, represent critical stages for evolution due to …

Apomixis and the paradox of sex in plants

E Hörandl - Annals of Botany, 2024 - academic.oup.com
Background The predominance of sex in eukaryotes, despite the high costs of meiosis and
mating, remains an evolutionary enigma. Many theories have been proposed, none of them …

Photoperiod Extension Enhances Sexual Megaspore Formation and Triggers Metabolic Reprogramming in Facultative Apomictic Ranunculus auricomus

S Klatt, F Hadacek, L Hodač, G Brinkmann… - Frontiers in Plant …, 2016 - frontiersin.org
Meiosis, the key step of sexual reproduction, persists in facultative apomictic plants
functional to some extent. However, it still remains unclear how and why proportions of …

A novel strategy to study apomixis, automixis, and autogamy in plants

P Šarhanová, Ľ Majeský, M Sochor - Plant Reproduction, 2024 - Springer
Key message The combination of a flow cytometric seed screen and genotyping of each
single seed offers a cost-effective approach to detecting complex reproductive pathways in …

Effects of apomixis and polyploidy on diversification and geographic distribution in Amelanchier (Rosaceae)

MB Burgess, KR Cushman, ET Doucette… - American Journal of …, 2014 - Wiley Online Library
• Premise of the study: Amelanchier polyploid apomicts differ from sexual diploids in their
more complex diversification, greater species problems, and geographic distribution. To …