Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobate Mnemiopsis leidyi

JS Presnell, M Bubel, T Knowles, W Patry… - Nature protocols, 2022 - nature.com
Despite long-standing experimental interest in ctenophores due to their unique biology,
ecological influence and evolutionary status, previous work has largely been constrained by …

[HTML][HTML] Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts

A Rosner, J Armengaud, L Ballarin… - Science of the Total …, 2021 - Elsevier
Environmental stressors are assessed through methods that quantify their impacts on a wide
range of metrics including species density, growth rates, reproduction, behaviour and …

Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

JD Nowotny, MT Connelly, N Traylor-Knowles - Scientific reports, 2021 - nature.com
Cnidarians are emerging model organisms for cell and molecular biology research.
However, successful cell culture development has been challenging due to incomplete …

Extracellular DNA traps in a ctenophore demonstrate immune cell behaviors in a non-bilaterian

LE Vandepas, C Stefani, PP Domeier… - Nature …, 2024 - nature.com
The formation of extracellular DNA traps (ETosis) is a first response mechanism by specific
immune cells following exposure to microbes. Initially characterized in vertebrate …

Brief history of Ctenophora

LL Moroz - Ctenophores: Methods and Protocols, 2024 - Springer
Ctenophores are the descendants of the earliest surviving lineage of ancestral metazoans,
predating the branch leading to sponges (Ctenophore-first phylogeny). Emerging genomic …

Independent innexin radiation shaped signaling in ctenophores

J Ortiz, YV Bobkov, MB DeBiasse… - Molecular Biology …, 2023 - academic.oup.com
Innexins facilitate cell–cell communication by forming gap junctions or nonjunctional
hemichannels, which play important roles in metabolic, chemical, ionic, and electrical …

Still Enigmatic: Innate Immunity in the Ctenophore Mnemiopsis leidyi

N Traylor-Knowles, LE Vandepas… - Integrative and …, 2019 - academic.oup.com
Innate immunity is an ancient physiological response critical for protecting metazoans from
invading pathogens. It is the primary pathogen defense mechanism among invertebrates …

Flow cytometry methods for targeted isolation of ctenophore cells

AC Dieter, ABK True, EA Gilbertson… - Frontiers in Marine …, 2023 - frontiersin.org
Cell suspension fluidics, such as flow cytometry (FCS) and fluorescence-activated cell
sorting (FACS), facilitates the identification and precise separation of individual cells based …

Sea urchin Paracentrotus lividus immune cells in culture: formulation of the appropriate harvesting and culture media and maintenance conditions

A Pinsino, A Alijagic - Biology open, 2019 - journals.biologists.com
The sea urchin is an emergent model system for studying basic and translational
immunology. Here we report a new method for the harvesting and maintenance of primary …

Cnidarian primary cell culture as a tool to investigate the effect of thermal stress at cellular level

P Ventura, G Toullec, C Fricano, L Chapron… - Marine …, 2018 - Springer
In the context of global change, symbiotic cnidarians are largely affected by seawater
temperature elevation leading to symbiosis breakdown. This process, also called bleaching …