Understanding gene function and regulation in homeostasis and disease requires
knowledge of the cellular and tissue contexts in which genes are expressed. Here, we …

Single-cell RNA-seq (scRNA-seq) has revolutionized modern genomics, but the large size of
myotubes and myofibers has restricted use of scRNA-seq in skeletal muscle. For the study of …

Key early features of amyotrophic lateral sclerosis (ALS) are denervation of neuromuscular
junctions and axonal degeneration. Motor neuron homeostasis relies on local translation …

Skeletal muscle fiber is a large syncytium with multiple and evenly distributed nuclei. Adult
subsynaptic myonuclei beneath the neuromuscular junction (NMJ) express specific genes …

Skeletal muscle myofibers have differential protein expression resulting in functionally
distinct slow-and fast-twitch types. While certain protein classes are well-characterized, the …