Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Stem cell separation technologies

B Zhu, SK Murthy - Current opinion in chemical engineering, 2013 - Elsevier
Stem cell therapy and translational stem cell research require large-scale supply of stem
cells at high purity and viability, thus leading to the development of stem cell separation …

[HTML][HTML] Microfluidic tools for enhanced characterization of therapeutic stem cells and prediction of their potential antimicrobial secretome

P Marrazzo, V Pizzuti, S Zia, A Sargenti, D Gazzola… - Antibiotics, 2021 - mdpi.com
Antibiotic resistance is creating enormous attention on the development of new antibiotic-
free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are …

[HTML][HTML] Interferon-gamma primed human clonal mesenchymal stromal cell sheets exhibit enhanced immunosuppressive function

CM Dunn, S Kameishi, YK Cho, SU Song, DW Grainger… - Cells, 2022 - mdpi.com
Mesenchymal stromal cells (MSCs) represent a promising treatment for immune-related
diseases due to their diverse immunomodulatory paracrine functions. However, progress of …

Cells as advanced therapeutics: State-of-the-art, challenges, and opportunities in large scale biomanufacturing of high-quality cells for adoptive immunotherapies

NJ Dwarshuis, K Parratt, A Santiago-Miranda… - Advanced drug delivery …, 2017 - Elsevier
Therapeutic cells hold tremendous promise in treating currently incurable, chronic diseases
since they perform multiple, integrated, complex functions in vivo compared to traditional …

Highly selective biomechanical separation of cancer cells from leukocytes using microfluidic ratchets and hydrodynamic concentrator

BK Lin, SM McFaul, C Jin, PC Black, H Ma - Biomicrofluidics, 2013 - pubs.aip.org
The separation of cells based on their biomechanical properties, such as size and
deformability, is important in applications such as the identification of circulating tumor cells …

[HTML][HTML] Field-Flow Fractionation in Molecular Biology and Biotechnology

S Giordani, V Marassi, A Placci, A Zattoni, B Roda… - Molecules, 2023 - mdpi.com
Field-flow fractionation (FFF) is a family of single-phase separative techniques exploited to
gently separate and characterize nano-and microsystems in suspension. These techniques …

Separation technologies for stem cell bioprocessing

MM Diogo, CL da Silva… - Biotechnology and …, 2012 - Wiley Online Library
Stem cells have been the focus of an intense research due to their potential in Regenerative
Medicine, drug discovery, toxicology studies, as well as for fundamental studies on …

[HTML][HTML] Effective label-free sorting of multipotent mesenchymal stem cells from clinical bone marrow samples

S Zia, C Cavallo, I Vigliotta, V Parisi, B Grigolo, R Buda… - Bioengineering, 2022 - mdpi.com
Mesenchymal stem cells (MSC) make up less than 1% of the bone marrow (BM). Several
methods are used for their isolation such as gradient separation or centrifugation, but these …

Analytical strategies for improving the robustness and reproducibility of bioluminescent microbial bioreporters

A Roda, B Roda, L Cevenini, E Michelini… - Analytical and …, 2011 - Springer
Whole-cell bioluminescent (BL) bioreporter technology is a useful analytical tool for
developing biosensors for environmental toxicology and preclinical studies. However, when …