Structures and strategies of anti-CRISPR-mediated immune suppression
T Wiegand, S Karambelkar… - Annual review of …, 2020 - annualreviews.org
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …
Role of computational methods in going beyond X-ray crystallography to explore protein structure and dynamics
Protein structural biology came a long way since the determination of the first three-
dimensional structure of myoglobin about six decades ago. Across this period, X-ray …
dimensional structure of myoglobin about six decades ago. Across this period, X-ray …
Characterization and repurposing of the endogenous Type IF CRISPR–Cas system of Zymomonas mobilis for genome engineering
Y Zheng, J Han, B Wang, X Hu, R Li… - Nucleic acids …, 2019 - academic.oup.com
Application of CRISPR-based technologies in non-model microorganisms is currently very
limited. Here, we reported efficient genome engineering of an important industrial …
limited. Here, we reported efficient genome engineering of an important industrial …
Structure reveals a mechanism of CRISPR-RNA-guided nuclease recruitment and anti-CRISPR viral mimicry
Bacteria and archaea have evolved sophisticated adaptive immune systems that rely on
CRISPR RNA (crRNA)-guided detection and nuclease-mediated elimination of invading …
CRISPR RNA (crRNA)-guided detection and nuclease-mediated elimination of invading …
CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus
K Yoshimi, K Takeshita, S Yamayoshi, S Shibumura… - Iscience, 2022 - cell.com
CRISPR-based diagnostics (CRISPR-dx), including the Cas12-based DETECTR and Cas13-
based SHERLOCK Class 2 CRISPRs, have been used to detect the presence of DNA or …
based SHERLOCK Class 2 CRISPRs, have been used to detect the presence of DNA or …
Mechanisms of Type IE and IF CRISPR-Cas Systems in Enterobacteriaceae
C Xue, DG Sashital - EcoSal plus, 2019 - Am Soc Microbiol
CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against
invasion by bacteriophages and other mobile genetic elements. Short fragments of invader …
invasion by bacteriophages and other mobile genetic elements. Short fragments of invader …
Characterization and repurposing of type I and type II CRISPR–Cas systems in bacteria
CRISPR–Cas systems constitute the adaptive immune system of bacteria and archaea, as a
sequence-specific nucleic acid targeting defense mechanism. The sequence-specific …
sequence-specific nucleic acid targeting defense mechanism. The sequence-specific …
Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3
Abstract Type I CRISPR-Cas3 uses an RNA-guided multi Cas-protein complex, Cascade,
which detects and degrades foreign nucleic acids via the helicase-nuclease Cas3 protein …
which detects and degrades foreign nucleic acids via the helicase-nuclease Cas3 protein …
Dynamics of hepatitis B virus capsid protein dimer regulate assembly through an allosteric network
While there is an effective vaccine for Human Hepatitis B Virus (HBV), 257 million people
have chronic infections for which there is no cure. The assembly process for the viral capsid …
have chronic infections for which there is no cure. The assembly process for the viral capsid …
Cas3 protein—a review of a multi-tasking machine
L He, M St. John James, M Radovcic, I Ivancic-Bace… - Genes, 2020 - mdpi.com
Cas3 has essential functions in CRISPR immunity but its other activities and roles, in vitro
and in cells, are less widely known. We offer a concise review of the latest understanding …
and in cells, are less widely known. We offer a concise review of the latest understanding …