Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

J González‐Valdez, K Mayolo‐Deloisa… - Journal of Chemical …, 2018 - Wiley Online Library
Traditionally, aqueous two‐phase systems (ATPS) have been used as a liquid–liquid
extraction technique for the primary recovery and purification of biological samples. The …

Economic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems

MA Torres-Acosta, JFB Pereira, MG Freire… - Separation and …, 2018 - Elsevier
Antibiotics are a key pharmaceutical to inhibit growth or kill microorganisms. They represent
a profitable market and, in particular, tetracycline has been listed as an essential medicine …

Characterization of polymer‐polymer aqueous two‐phase system droplets for 3D culture future applications

K Chairez‐Cantu… - Journal of Chemical …, 2023 - Wiley Online Library
BACKGROUND Cell‐based high‐throughput platforms using aqueous two‐phase systems
(ATPS) have led to the construction of 3D cultures using ATPS in which stem cells are …

General concepts and definitions of aqueous two-phase systems

K Mayolo-Deloisa, J Benavides… - Aqueous Two-Phase …, 2017 - Springer
Aqueous two-phase systems (ATPS) are biphasic systems composed, traditionally, by two
polymers or one polymer and one salt. However, the classification currently includes ATPS …

Aqueous two-phase system formation in small droplets by shirasu porous glass membrane emulsification followed by water extraction

K Akamatsu, R Kurita, D Sato, S Nakao - Langmuir, 2019 - ACS Publications
By utilizing water transport phenomena between two different water-in-oil (W/O) emulsion
droplets through continuous oil phase, we developed a novel method of aqueous two-phase …

Enrichment and recovery of mammalian cells from contaminated cultures using aqueous two-phase systems

CJ Luby, BP Coughlin, CR Mace - Analytical chemistry, 2018 - ACS Publications
This Article describes a density-based method for removing contaminants, including
microorganisms and nonviable cells, from mammalian cell cultures using an aqueous two …

Influence of tie line length and volume ratio on the partition behavior of peripheral blood and conjugated CD34 antibody in polymer-polymer aqueous two-phase …

M Nisslein, M González-González… - Separation and …, 2021 - Elsevier
Aqueous two-phase systems (ATPS) offer great opportunities regarding the purification of
stem cells for future medical applications. However, most publications exploring the use of …

Aqueous two‐phase systems as a novel alternative in the primary recovery of human mesenchymal cells obtained from adipose tissue

D Freire‐Flores, N Llanovarced‐Kawles… - Journal of Chemical …, 2024 - Wiley Online Library
In recent years, therapies based on regenerative medicine have gained wide interest owing
to their potential in organ transplantation and in the treatment of multiple diseases. These …

Potential of aqueous two‐phase systems for the separation of levodopa from similar biomolecules

RCS Sousa, CMSS Neves, MM Pereira… - Journal of Chemical …, 2018 - Wiley Online Library
BACKGROUND Levodopa is a precursor of several neurotransmitters, such as dopamine,
and is used in the treatment of Parkinson's disease. In this work, an alternative strategy was …