Innovations to culturing the uncultured microbial majority

WH Lewis, G Tahon, P Geesink, DZ Sousa… - Nature Reviews …, 2021 - nature.com
Despite the surge of microbial genome data, experimental testing is important to confirm
inferences about the cell biology, ecological roles and evolution of microorganisms. As the …

Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

Challenges of unculturable bacteria: environmental perspectives

A Bodor, N Bounedjoum, GE Vincze… - … Science and Bio …, 2020 - Springer
Environmental biotechnology offers several promising techniques for the rehabilitation of
polluted environments. The modern industrialized world presents novel challenges to the …

[HTML][HTML] The human microbiota in health and disease

B Wang, M Yao, L Lv, Z Ling, L Li - Engineering, 2017 - Elsevier
Trillions of microbes have evolved with and continue to live on and within human beings. A
variety of environmental factors can affect intestinal microbial imbalance, which has a close …

Growing unculturable bacteria

EJ Stewart - Journal of bacteriology, 2012 - Am Soc Microbiol
The bacteria that can be grown in the laboratory are only a small fraction of the total diversity
that exists in nature. At all levels of bacterial phylogeny, uncultured clades that do not grow …

Oligotyping: differentiating between closely related microbial taxa using 16S rRNA gene data

AM Eren, L Maignien, WJ Sul… - Methods in ecology …, 2013 - Wiley Online Library
Bacteria comprise the most diverse domain of life on Earth, where they occupy nearly every
possible ecological niche and play key roles in biological and chemical processes. Studying …

Present and future of culturing bacteria

J Overmann, B Abt, J Sikorski - Annual review of microbiology, 2017 - annualreviews.org
The cultivation of bacteria is highly biased toward a few phylogenetic groups. Many of the
currently underexplored bacterial lineages likely have novel biosynthetic pathways and …

Strategies for culture of 'unculturable'bacteria

SR Vartoukian, RM Palmer… - FEMS microbiology …, 2010 - academic.oup.com
Molecular ecology methods are now well established for the culture-independent
characterization of complex bacterial communities associated with various environmental …

Use of Ichip for High-Throughput In Situ Cultivation of “Uncultivable” Microbial Species

D Nichols, N Cahoon, EM Trakhtenberg… - Applied and …, 2010 - Am Soc Microbiol
One of the oldest unresolved microbiological phenomena is why only a small fraction of the
diverse microbiological population grows on artificial media. The “uncultivable” microbial …

Metagenomics: application of genomics to uncultured microorganisms

J Handelsman - Microbiology and molecular biology reviews, 2004 - Am Soc Microbiol
Metagenomics (also referred to as environmental and community genomics) is the genomic
analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of …